Xiao Chengfeng, Qiu Shuang, Robertson R Meldrum
Department of Biology, Queen's University, Kingston, ON, K7L 3N6, Canada.
Behav Genet. 2018 Jan;48(1):80-93. doi: 10.1007/s10519-017-9881-z. Epub 2017 Nov 2.
We describe persistent one-way walking of Drosophila melanogaster in a circular arena. Wild-type Canton-S adult flies walked in one direction, counter-clockwise or clockwise, for minutes, whereas white-eyed mutant [Formula: see text] changed directions frequently. Locomotion in the circular arena could be classified into four components: counter-clockwise walking, clockwise walking, nondirectional walking and pausing. Genetic analysis revealed that while wild-type genetic background was associated with reduced directional change and reduced numbers of one-way (including counter-clockwise and clockwise) and nondirectional walks, the white ([Formula: see text]) locus promoted persistent one-way walking by increasing the maximal duration of one-way episodes. The promoting effect of [Formula: see text] was further supported by the observations that (1) [Formula: see text] duplicated to the Y chromosome, (2) four genomic copies of mini-white inserted on the autosomes, and (3) pan-neuronal overexpression of the White protein increased the maximal duration of one-way episodes, and that RNAi knockdown of [Formula: see text] in the neurons decreased the maximal duration of one-way episodes. These results suggested a pleiotropic function of [Formula: see text] in promoting persistent one-way walking in the circular arena.
我们描述了黑腹果蝇在圆形场地中的持续单向行走。野生型Canton-S成年果蝇会朝一个方向，逆时针或顺时针行走数分钟，而白眼突变体[公式：见正文]则频繁改变方向。在圆形场地中的运动可分为四个部分：逆时针行走、顺时针行走、无方向行走和暂停。遗传分析表明，野生型遗传背景与方向变化减少以及单向（包括逆时针和顺时针）和无方向行走次数减少有关，而白眼（[公式：见正文]）基因座通过增加单向行走的最长持续时间来促进持续单向行走。[公式：见正文]的促进作用还得到以下观察结果的进一步支持：（1）[公式：见正文]复制到Y染色体上，（2）四个迷你白眼基因组拷贝插入常染色体，（3）泛神经元过表达White蛋白增加了单向行走的最长持续时间，而神经元中[公式：见正文]的RNA干扰敲低则减少了单向行走的最长持续时间。这些结果表明[公式：见正文]在促进圆形场地中持续单向行走方面具有多效性功能。
