Department of Stomatology, Faculty of Health Sciences, URJC, 28922, Alcorcon-Madrid, Spain.
Department of Physical-Chemistry II, Faculty of Pharmacy, Complutense University of Madrid, 28040, Madrid, Spain.
J Biomed Mater Res B Appl Biomater. 2018 Aug;106(6):2307-2315. doi: 10.1002/jbm.b.34040. Epub 2017 Nov 3.
Porous ceramics doped with silicon and pure β-TCP were analyzed in terms of internal microstructure, cell behavior, and the percentage of newly formed bone. Additionally the materials were tested to determine which of the two had better properties to load and release vancomycin hydrochloride. Internal pore distribution and porosity were determined through high pressure mercury porosimetry and the specific surface area was measured by the Brunauer Emmet-Teller method. The proliferation and viability of the human osteoblast-like cell line MG-63 was studied to validate both materials. The materials were tested on eight New Zealand rabbits which created defects, 10 mm in diameter, in the calvaria bone. After 8 and 12 weeks a histological and histomorphometric analysis was performed. Si-β-TCP showed a higher porosity and specific surface area. The cytocompatibility test revealed acceptable results in terms of proliferation and viability whereas the percentage of new bone was higher in Si-β-TCP with a two-time study being statistically significant with 12 weeks of healing (p < 0.05).The vancomycin loaded within the ceramic scaffolds were burst released and the material had the ability to inhibit bacterial growth. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2307-2315, 2018.
多孔陶瓷掺杂硅和纯 β-TCP，从内部微观结构、细胞行为和新形成骨的百分比等方面进行了分析。此外，还对这两种材料进行了测试，以确定哪一种材料具有更好的负载和释放盐酸万古霉素的性能。通过高压汞孔隙率法测定了内部孔隙分布和孔隙率，通过 Brunauer Emmet-Teller 方法测定了比表面积。通过人成骨样细胞系 MG-63 研究了这两种材料的增殖和活力。将这两种材料用于 8 只新西兰白兔，在颅骨上造成 10mm 直径的缺损。8 周和 12 周后，进行组织学和组织形态计量学分析。Si-β-TCP 具有更高的孔隙率和比表面积。细胞相容性试验显示，在增殖和活力方面具有可接受的结果，而在 Si-β-TCP 中，新骨的百分比更高，12 周愈合时具有统计学意义（p<0.05）。载于陶瓷支架内的万古霉素呈爆发式释放，该材料具有抑制细菌生长的能力。© 2017 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料，106B：2307-2315，2018 年。
