Influence of DEHP on thyroid, sex steroid-related genes and gonadal differentiation in Rana chensinensis tadpoles.

In the present study, responses of the Chinese brown frog (Rana chensinensis) to exposure to di-2-ethylhexyl phthalate (DEHP), a common plasticizer, during the larval period were characterized. The effects of DEHP on metamorphosis rate, thyroid hormone, thyroid histology and the expression of genes involved in the steroid hormone synthesis in gonad were investigated. Metamorphosis rate and 50 percent of the tadpoles to reach Gosner stage 42 (T ) were significantly slower in all DEHP groups. The thyroid glands of the tadpoles exposed to DEHP clearly exhibited colloid depletion. In addition, decreased concentrations of T4 and T3 were observed in the tadpoles exposed to DEHP. Moreover, the highest DEHP exposure (10 µmol/L DEHP) showed increased ratio of females significantly. Also, up-regulation significantly of transcripts of cytochrome P450 aromatase (CYP19) gene was detected in male tadpoles exposed to DEHP. The present results indicate that this increase in estrogens could lead to female-biased sex ratio in DEHP exposure group. Taken together, the present study indicates that DEHP disrupt thyroid hormone and sex steroid signaling in R. chensinensis tadpoles. Our present observations support evidence of a crosstalk between TH and sex steroids in gonad differentiation.