College of Life Sciences, Shaanxi Normal University, Xi'an 710119, Shaanxi, PR China.
College of Life Sciences, Shaanxi Normal University, Xi'an 710119, Shaanxi, PR China.
Ecotoxicol Environ Saf. 2019 Mar;169:747-755. doi: 10.1016/j.ecoenv.2018.11.083. Epub 2018 Nov 28.
Octylphenol (OP), a class of endocrine disrupting chemicals (EDCs), could produce adverse effects on developmental process of animals. Thyroid hormone is one of the important hormones involved in animal development. To determine whether OP affect the growth and development of amphibian larvae via interfering the thyroid function, Rana chensinensis larvae at Gosner stage 29 were exposed to 10, 10 and 10 mol/L OP in the present study. Results demonstrated that OP could decrease the body length and mass and retard the development of tadpoles. The histologic evaluation showed microscopic structures of thyroid gland were changed in 10 and 10 mol/L OP treated groups on day 40. The expression levels of Dio2, Dio3, TRα and TRβ mRNA in the liver, brain, skin and tail of tadpoles were detected by qRT-PCR, when treated with OP for 20, 30, 40 and 50 day, respectively. The results of qRT-PCR showed OP could affect the expressions of Dio2, Dio3, TRα and TRβ mRNA in the four tissues, and then influence the activity and function of THs, further affecting the growth and development of the tadpoles.
辛基酚（OP）是一类内分泌干扰化学物质（EDCs），可能对动物的发育过程产生不良影响。甲状腺激素是参与动物发育的重要激素之一。为了确定 OP 是否通过干扰甲状腺功能影响两栖动物幼虫的生长和发育，本研究将处于 Gosner 29 期的中华大蟾蜍（Rana chensinensis）幼虫暴露于 10、10 和 10 mol/L 的 OP 中。结果表明，OP 可降低幼虫的体长和体重，延缓其发育。组织学评价显示，在 10 和 10 mol/L OP 处理组中，第 40 天甲状腺的微观结构发生了变化。通过 qRT-PCR 检测了 OP 处理 20、30、40 和 50 天的幼虫肝脏、大脑、皮肤和尾巴中 Dio2、Dio3、TRα 和 TRβ mRNA 的表达水平。qRT-PCR 的结果表明，OP 可影响四个组织中 Dio2、Dio3、TRα 和 TRβ mRNA 的表达，从而影响 THs 的活性和功能，进一步影响幼体的生长和发育。
