Hong Jin-Ni, Li Wei-Wei, Fu Hong, Wang Xue-Mei
Integrated Laboratory of Traditional Chinese Medicine and Western Medicine, Peking University, First Hospital, Beijing 100034, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jul;42(14):2754-2759. doi: 10.19540/j.cnki.cjcmm.20170609.007.
To elucidate the efficacy of Jiangtang decoction(JTD) on AGEs-RAGE and oxidative stress in type 2 diabetic model KK-Ay mice. Fifty KK-Ay mice were randomly divided into 5 groups as follows： model group, metformin group, low-dose, medium-dose and high-dose of JTD group, with 10 C57BL/6J as normal group. All groups are orally administrated with equal distilled water, 250 mg•kg⁻¹ metformin hydrochloride, 2, 4,8 g•kg⁻¹ JTD, equal distilled water respectively, once per day for 12 weeks. Alanine aminotransferase(ALT), creatinine(CREA), urea nitrogen(BUN),advanced glycation end products(AGEs) and receptor of glycation end products(RAGE) in blood or urine were measured during the experiments. Furthermore, on the day of the sacrifice, kidney was collected, and electronic microscopy and immunohistochemistry were performed to evaluate the protective renal effect of JTD. In addition, the levels of AGEs, RAGE, Cata-lase(CAT) and superoxide dismutase(SOD) were assessed by Western blot, Real-time PCR or ELISA to analyze the efficacy of JTD. This study demonstrated that JTD might protect kidney of KK-Ay by down-regulating the expression of AGEs, RAGE and oxidative stress.
为阐明降糖汤（JTD）对2型糖尿病模型KK-Ay小鼠晚期糖基化终产物（AGEs）-糖基化终产物受体（RAGE）及氧化应激的影响。将50只KK-Ay小鼠随机分为5组：模型组、二甲双胍组、JTD低剂量组、中剂量组和高剂量组，另取10只C57BL/6J小鼠作为正常组。所有组分别给予等体积蒸馏水、250 mg•kg⁻¹盐酸二甲双胍、2、4、8 g•kg⁻¹ JTD、等体积蒸馏水，每日1次，连续12周。实验期间检测血液或尿液中的谷丙转氨酶（ALT）、肌酐（CREA）、尿素氮（BUN）、晚期糖基化终产物（AGEs）和糖基化终产物受体（RAGE）。此外，在处死当天，取肾脏进行电子显微镜检查和免疫组织化学分析，以评估JTD对肾脏的保护作用。另外，通过蛋白质免疫印迹法、实时荧光定量PCR或酶联免疫吸附测定法评估AGEs、RAGE、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平，以分析JTD的疗效。本研究表明，JTD可能通过下调AGEs、RAGE的表达及氧化应激来保护KK-Ay小鼠的肾脏。
