源自多酚的食品添加剂对金黄色葡萄球菌产生葡萄球菌肠毒素A及形成生物膜的抑制作用。
Inhibitory effects of food additives derived from polyphenols on staphylococcal enterotoxin A production and biofilm formation by Staphylococcus aureus.
作者信息
Shimamura Yuko, Hirai Chikako, Sugiyama Yuka, Shibata Masaharu, Ozaki Junya, Murata Masatsune, Ohashi Norio, Masuda Shuichi
机构信息
a School of Food and Nutritional Sciences , University of Shizuoka , Shizuoka , Japan.
b Department of Nutrition and Food Science , Ochanomizu University , Tokyo , Japan.
出版信息
Biosci Biotechnol Biochem. 2017 Dec;81(12):2346-2352. doi: 10.1080/09168451.2017.1395681. Epub 2017 Nov 3.
In this study, we examined the inhibitory effects of 14 food additives derived from polyphenol samples on staphylococcal enterotoxin A (SEA) production and biofilm formation by Staphylococcus aureus. Tannic acid AL (TA), Purephenon 50 W (PP) and Polyphenon 70A (POP) at 0.25 mg/mL and Gravinol®-N (GN), Blackcurrant polyphenol AC10 (BP), and Resveratrol-P5 (RT) at 1.0 mg/mL significantly decreased SEA production by S. aureus C-29 (p < 0.05). TA, GN, BP, and RT significantly inhibited the expression of the sea gene in S. aureus C-29 (p < 0.05), while suppression attempts by PP and POP proved unsuccessful. After result analysis, it can be derived that TA, GN, BP, and RT inhibit the production of SEA. Of the six samples, each one significantly inhibited biofilm formation (p < 0.05). Food additives derived from polyphenols have viability to be used as a means to inhibit the enterotoxin production and control the biofilm formation of foodborne pathogens.
在本研究中，我们检测了14种源自多酚样品的食品添加剂对金黄色葡萄球菌产生葡萄球菌肠毒素A（SEA）及形成生物膜的抑制作用。0.25mg/mL的单宁酸AL（TA）、纯酚50W（PP）和多酚70A（POP），以及1.0mg/mL的Gravinol®-N（GN）、黑加仑多酚AC10（BP）和白藜芦醇-P5（RT）显著降低了金黄色葡萄球菌C-29的SEA产量（p<0.05）。TA、GN、BP和RT显著抑制了金黄色葡萄球菌C-29中sea基因的表达（p<0.05），而PP和POP的抑制尝试未成功。经过结果分析，可以得出TA、GN、BP和RT抑制SEA产生的结论。在这六个样品中，每一个都显著抑制了生物膜的形成（p<0.05）。源自多酚的食品添加剂有潜力用作抑制食源性病原体肠毒素产生及控制生物膜形成的手段。
Zotero 插件