一种用于检测次氯酸的快速响应荧光探针及其在活细胞外源性和内源性次氯酸成像中的应用。
A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells.
作者信息
Jiang Yin, Zheng Guansheng, Cai Ning, Zhang Huatang, Tan Yi, Huang Mengjiao, He Yonghe, He Jun, Sun Hongyan
机构信息
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, Guangdong 510006, China.
出版信息
Chem Commun (Camb). 2017 Nov 14;53(91):12349-12352. doi: 10.1039/c7cc07373a.
A facile fluorescent probe (NBD-DOP) has been developed to detect hypochlorous acid (HOCl) in this study. The probe consists of a NBD fluorophore and a dopamine moiety that reacts with HOCl specifically. The dopamine group quenches the fluorescence of NBD efficiently through a photoinduced electron transfer (PET) effect. Experimental data showed that NBD-DOP could detect HOCl with ultrafast response, high sensitivity and high selectivity in a wide pH range. The probe could also be used to detect the myeloperoxidase enzyme that produces HOCl. Moreover, NBD-DOP has been applied in the imaging of exogenous and endogenous HOCl in living cells by confocal fluorescence microscopy.
在本研究中，已开发出一种简便的荧光探针（NBD-DOP）用于检测次氯酸（HOCl）。该探针由一个NBD荧光团和一个与HOCl特异性反应的多巴胺部分组成。多巴胺基团通过光诱导电子转移（PET）效应有效地淬灭了NBD的荧光。实验数据表明，NBD-DOP能够在很宽的pH范围内以超快响应、高灵敏度和高选择性检测HOCl。该探针还可用于检测产生HOCl的髓过氧化物酶。此外，NBD-DOP已通过共聚焦荧光显微镜应用于活细胞中外源和内源HOCl的成像。
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