Jelenc P C, Kurland C G
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3174-8. doi: 10.1073/pnas.76.7.3174.
The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) reduced further the missense error frequency to the in vivo level as well as enhanced the extent of translation. The effect of nucleoside triphosphate regeneration was shown to be due primarily to the increase in the ratio of adenosine and guanosine triphosphates to their hydrolysis products and only marginally due to the increase in the absolute concentrations of the nucleoside triphosphates.
向体外蛋白质合成系统中添加天然存在的多胺和无机离子，提高了翻译的程度和保真度。在这样一个优化的系统中，用磷酸烯醇丙酮酸和丙酮酸激酶（ATP：丙酮酸2-O-磷酸转移酶，EC 2.7.1.40）再生核苷三磷酸，进一步将错义错误频率降低到体内水平，并提高了翻译程度。核苷三磷酸再生的作用主要是由于腺苷三磷酸和鸟苷三磷酸与其水解产物的比例增加，而核苷三磷酸绝对浓度的增加仅起很小的作用。
