Université de Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, F-67000 Strasbourg, France.
Genes (Basel). 2021 Jul 24;12(8):1125. doi: 10.3390/genes12081125.
RNA modifications are involved in numerous biological processes and are present in all RNA classes. These modifications can be constitutive or modulated in response to adaptive processes. RNA modifications play multiple functions since they can impact RNA base-pairings, recognition by proteins, decoding, as well as RNA structure and stability. However, their roles in stress, environmental adaptation and during infections caused by pathogenic bacteria have just started to be appreciated. With the development of modern technologies in mass spectrometry and deep sequencing, recent examples of modifications regulating host-pathogen interactions have been demonstrated. They show how RNA modifications can regulate immune responses, antibiotic resistance, expression of virulence genes, and bacterial persistence. Here, we illustrate some of these findings, and highlight the strategies used to characterize RNA modifications, and their potential for new therapeutic applications.
RNA 修饰参与众多生物过程，并存在于所有 RNA 类别中。这些修饰可以是组成性的，也可以响应适应性过程而被调节。RNA 修饰具有多种功能，因为它们可以影响 RNA 的碱基配对、蛋白质的识别、解码以及 RNA 的结构和稳定性。然而，它们在应激反应、环境适应以及由致病性细菌引起的感染过程中的作用才刚刚开始被认识。随着质谱和深度测序等现代技术的发展，最近已经有一些修饰调节宿主-病原体相互作用的例子被证明。这些例子表明，RNA 修饰如何调节免疫反应、抗生素耐药性、毒力基因的表达和细菌的持久性。在这里，我们举例说明了其中的一些发现，并强调了用于表征 RNA 修饰的策略及其在新的治疗应用中的潜力。
