College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
PLoS One. 2017 Nov 3;12(11):e0187622. doi: 10.1371/journal.pone.0187622. eCollection 2017.
In this study, the effects of coronatine treatment on the growth, comprehensive metabolic profiles, and productivity of bioactive compounds, including phenolics and phytosterols, in whole plant cultures of Lemna paucicostata were investigated using gas chromatography-mass spectrometry (GC-MS) coupled with multivariate statistical analysis. To determine the optimal timing of coronatine elicitation, coronatine was added on days 0, 23, and 28 after inoculation. The total growth of L. paucicostata was not significantly different between the coronatine treated groups and the control. The coronatine treatment in L. paucicostata induced increases in the content of hydroxycinnamic acids, such as caffeic acid, isoferulic acid, ρ-coumaric acid, sinapic acid, and phytosterols, such as campesterol and β-sitosterol. The productivity of these useful metabolites was highest when coronatine was added on day 0 and harvested on day 32. These results suggest that coronatine treatment on day 0 activates the phenolic and phytosterol biosynthetic pathways in L. paucicostata to a greater extent than in the control. To the best of our knowledge, this is the first report to investigate the effects of coronatine on the alteration of metabolism in L. paucicostata based on GC-MS profiling. The results of this research provide a foundation for designing strategies for enhanced production of useful metabolites for pharmaceutical and nutraceutical industries by cultivation of L. paucicostata.
在这项研究中，使用气相色谱-质谱联用（GC-MS）结合多变量统计分析，研究了冠菌素处理对浮萍（Lemna paucicostata）整体植物培养物生长、综合代谢谱和生物活性化合物（包括酚类和植物固醇）生产力的影响。为了确定冠菌素激发的最佳时机，在接种后第 0、23 和 28 天添加冠菌素。冠菌素处理组与对照组浮萍的总生长无显著差异。冠菌素处理诱导浮萍中羟基肉桂酸（如咖啡酸、异阿魏酸、ρ-香豆酸、芥子酸）和植物固醇（如菜油固醇、β-谷固醇）含量增加。当冠菌素在第 0 天添加并在第 32 天收获时，这些有用代谢物的生产力最高。这些结果表明，与对照组相比，冠菌素在第 0 天处理更能激活浮萍中酚类和植物固醇的生物合成途径。据我们所知，这是首次基于 GC-MS 图谱研究冠菌素对浮萍代谢改变的影响的报告。本研究结果为通过浮萍培养设计用于制药和营养保健品行业的有用代谢物增强生产策略提供了基础。
