He Libo, Wang Hao, Luo Lifei, Li Yongming, Huang Rong, Liao Lanjie, Zhu Zuoyan, Wang Yaping
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Oncotarget. 2017 Jul 22;8(44):76408-76422. doi: 10.18632/oncotarget.19460. eCollection 2017 Sep 29.
Bid, BH3-interacting domain death agonist, is a pro-apoptotic BH3-only member of Bcl-2 family, playing an important role in apoptosis. In the study, genes from grass carp () and rare minnow (), named and , were cloned and analyzed. was constitutively expressed in all examined tissues of grass carp, but the expression level varied in different tissues. Following grass carp reovirus (GCRV) stimulation , and apoptosis related genes and was up-regulated significantly at the late stage of infection. Moreover, we generated a -deficient rare minnow () to investigate the possible role of in GCRV-triggered apoptosis. We found that the survival time of rare minnow after GCRV infection was extended when compared with wild-type fish, the relative copy number of GCRV in rare minnow was lower than that in wild-type fish, and the expression level of and in rare minnow were significantly lower than that in the wild-type fish. Collectively, the current data revealed the important role of during virus-induced apoptosis in teleost fish. Our study would provide new insight into understanding the GCRV induced apoptosis and may provide a target gene for virus-resistant breeding in grass carp.
Bid，即BH3相互作用结构域死亡激动剂，是Bcl-2家族中仅含BH3结构域的促凋亡成员，在细胞凋亡中起重要作用。在本研究中，克隆并分析了草鱼（）和稀有鮈鲫（）的基因，分别命名为和。在草鱼的所有检测组织中均组成性表达，但在不同组织中的表达水平有所差异。在草鱼呼肠孤病毒（GCRV）刺激后，和凋亡相关基因和在感染后期显著上调。此外，我们构建了缺失的稀有鮈鲫（），以研究在GCRV触发的细胞凋亡中的可能作用。我们发现，与野生型鱼相比，GCRV感染后的稀有鮈鲫存活时间延长，稀有鮈鲫中GCRV的相对拷贝数低于野生型鱼，且稀有鮈鲫中和的表达水平显著低于野生型鱼。总体而言，目前的数据揭示了在硬骨鱼病毒诱导的细胞凋亡中的重要作用。我们的研究将为理解GCRV诱导的细胞凋亡提供新的见解，并可能为草鱼抗病毒育种提供一个靶基因。
