Impact of microRNA-34a and polymorphism of its target gene on susceptibility to uterine cervical cancer.

The purposes of this study were to associate the genetic polymorphisms in carbonic anhydrase 9 with uterine cervical cancer and identify the clinical implications. Three single-nucleotide polymorphisms (SNPs), rs2071676 (+201, G/A), rs3829078 (+1081, A/G), and rs1048638 (+1584, C/A), and an 18-base-pair deletion/insertion (376del393) in were examined. We used the Boyden chamber assay to evaluate the influence of on the migration of cervical cancers. Tissue microarrays were used to evaluate CAIX immunoreactivity and determine its clinical significance. The results revealed that the SNP rs1048638 is the only significant polymorphism that increases the risk of cervical cancer in Taiwanese women. We discovered that the SNP rs1048638 influences the expression of through the interaction between the 3'-untranslated region (UTR) of exon 11, where the SNP is located, and miR-34a, and influences the migration of cervical cancer cells. Moreover, we demonstrated that CAIX immunoreactivity is related to the occurrence of cervical cancer, and elevated CAIX immunoreactivity is associated with a more advanced stage. In conclusion, the finding that the SNP rs1048638 exerts its action through duplexes of the miR-34a and 3'-UTRs and plays a vital role in cervical cancer in Taiwanese women may be applicable to translational medicine.