State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China; Shanghai Institute of Technology, Shanghai 201418, China.
Microb Pathog. 2017 Dec;113:396-402. doi: 10.1016/j.micpath.2017.10.054. Epub 2017 Oct 31.
The antibacterial effect and mechanism of eugenol from Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf essential oil (CLEO) against oral anaerobe Porphyromonas gingivalis were investigated. The results showed that eugenol, with content of 90.84% in CLEO, exhibited antibacterial activity against P. gingivalis at a concentration of 31.25 μM. Cell shrink and lysis caused by eugenol were observed with Scanning Electron Microscopy (SEM). The release of macromolecules and uptake of fluorescent dye indicated that the antibacterial activity was due to the ability of eugenol to permeabilize the cell membrane and destroy the integrity of plasmatic membrane irreversibly. In addition, eugenol inhibited biofilm formation and reduced preformed biofilm of P. gingivalis at different concentrations. The down-regulation of virulence factor genes related to biofilm (fimA, hagA, hagB, rgpA, rgpB, kgp) explained that eugenol suppressed biofilm formation at the initial stage. These findings suggest that eugenol and CLEO may be potential additives in food and personal healthcare products as a prophylactic approach to periodontitis.
研究了丁香叶精油（CLEO）中含量为 90.84%的丁香酚对口腔厌氧菌牙龈卟啉单胞菌的抗菌作用及机制。结果表明，丁香酚在 31.25 μM 浓度下对牙龈卟啉单胞菌表现出抗菌活性。扫描电子显微镜（SEM）观察到丁香酚导致细胞收缩和裂解。大分子的释放和荧光染料的摄取表明，抗菌活性是由于丁香酚能够穿透细胞膜并不可逆地破坏质膜的完整性。此外，丁香酚在不同浓度下抑制牙龈卟啉单胞菌生物膜的形成和减少已形成的生物膜。与生物膜相关的毒力因子基因（fimA、hagA、hagB、rgpA、rgpB、kgp）的下调表明，丁香酚在初始阶段抑制生物膜的形成。这些发现表明，丁香酚和 CLEO 可能是食品和个人保健产品的潜在添加剂，可作为预防牙周炎的一种方法。
