Sawatphakdee Gotchagorn, Yostawonkul Jakarwan, Oontawee Saranyou, Rodprasert Watchareewan, Sawangmake Chenphop, Kornsuthisopon Chatvadee, Yata Teerapong, Tabtieang Sirinun Pisamai, Nowwarote Nunthawan, Pirarat Nopadon
Center of Excellence in Wildlife, Exotic, and Aquatic Animal Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand.
Animals (Basel). 2024 Jul 17;14(14):2084. doi: 10.3390/ani14142084.
Nanostructured lipid carriers (NLC) represent the second generation of nanoparticles, offering numerous advantages over conventional delivery systems. These include improved stability, enhanced drug-loading capacity, and controlled release profiles, making them highly attractive candidates for a wide range of therapeutic applications. Their suitability for hydrophobic drugs like a traditional medicinal plant of Thailand as clove oil and alpha-mangostin. We investigated into nanostructured lipid carriers loaded with Alpha-Mangostin and clove oil (NLC-AMCO) into the physicochemical and biological characteristics to identify the formulation with the highest efficacy for treatment. The particle size, charge, polydispersity index, and other characterizations were recorded. The realtime ex vivo penetration was explored using canine gingival tissue. Drug sustained release was assessed by HPLC. Moreover, the antibacterial properties were tested by conventional methods. The NLC-AMCO can be stored at up to 40 °C for 60 days without any alterations in particle characteristics. Gingival tissue penetration and sustained drug release were superior compared to unencapsulated counterparts. It exhibited greater effectiveness in inhibiting bacterial growth than the antibiotics tested, particularly against bacteria from the oral cavities of dogs. Therefore, this alternative treatment approach offers cost-effectiveness and ease of administration for pet owners and reduces discomfort for the animals during restraint.
纳米结构脂质载体（NLC）代表了第二代纳米颗粒，与传统给药系统相比具有众多优势。这些优势包括提高稳定性、增强载药能力和可控的释放曲线，使其成为广泛治疗应用中极具吸引力的候选者。它们适用于疏水性药物，如泰国传统药用植物丁香油和α-山竹黄酮。我们研究了负载α-山竹黄酮和丁香油的纳米结构脂质载体（NLC-AMCO）的物理化学和生物学特性，以确定治疗效果最佳的制剂。记录了粒径、电荷、多分散指数和其他特性。使用犬牙龈组织探索了实时离体渗透。通过高效液相色谱法评估药物的持续释放。此外，通过传统方法测试了抗菌性能。NLC-AMCO可在高达40°C的温度下储存60天，颗粒特性无任何变化。与未封装的对应物相比，牙龈组织渗透和药物持续释放更优。它在抑制细菌生长方面比测试的抗生素表现出更高的有效性，特别是对来自犬口腔的细菌。因此，这种替代治疗方法为宠物主人提供了成本效益和给药便利性，并减少了动物在约束过程中的不适感。
