Kosower N S, Kosower E M, Newton G L, Ranney H M
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3382-6. doi: 10.1073/pnas.76.7.3382.
The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and dibromobimane are effective on intact cells while red cell membranes may be impermeable to the positively charged monobromotrimethylammoniobimane, the latter being effective only on lysed cells. These bimane labels provide a class of labeling agents that may have wide applicability in biological materials.
双马来荧光标记物，一溴双马来、二溴双马来和一溴三甲基铵双马来，是顺式-9,10-二氧杂双马来：1,5-二氮杂双环[3.3.0]辛-3,6-二烯-2,8-二酮的衍生物。它们能在生理条件下有效地标记正常人红细胞中的血红蛋白（反应性巯基）、膜蛋白和谷胱甘肽。一溴双马来和二溴双马来对完整细胞有效，而带正电荷的一溴三甲基铵双马来可能无法透过红细胞膜，后者仅对裂解细胞有效。这些双马来标记物提供了一类可能在生物材料中具有广泛适用性的标记剂。
