Pandey Garima, Khatoon Sayyada, Pandey M M, Rawat A K S
Pharmacognosy & Ethnopharmacology Division, CSIR- National Botanical Research Institute, Lucknow- Post Box No. 436, Rana Pratap Marg, Lucknow, 226001, India.
Pharmacognosy & Ethnopharmacology Division, CSIR- National Botanical Research Institute, Lucknow- Post Box No. 436, Rana Pratap Marg, Lucknow, 226001, India.
J Ayurveda Integr Med. 2018 Jul-Sep;9(3):169-176. doi: 10.1016/j.jaim.2017.02.010.
The quality of herbal medicine is determined by its secondary metabolites, which may vary according to growth, season and altitude etc. OBJECTIVE: We studied the variation in phytochemistry and biological activities of Thalictrum foliolosum (TF) roots collected from four sites at different altitudes.
The berberine content in different extracts of T. foliolosum roots collected from various altitudes was estimated using HPTLC. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau reagent and aluminum chloride method respectively. The sensitivity of microbes for the extracts was studied using disc diffusion and the MIC was estimated using broth dilution method. Antioxidant capacity of the plant was studied using β-carotene bleaching assay, lipid peroxidation assay using goat liver, reducing power assay and DPPH free radical scavenging activity.
Berberine content varied inversely with altitude; while phenol and flavonoid content of TF increased at higher altitudes. All the TF extracts showed moderate to high activity against Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Extracts with high berberine content were most effective against C. albicans and S. aureus and also showed relatively significant anti-lipid peroxidation, β-carotene bleaching and reducing power. TF extracts with higher phenol and flavonoid content showed better scavenging of DPPH free radicals. Berberine was used as a standard in all the antioxidant and antimicrobial experiments performed.
Thalictrum from lower elevations can be explored as an alternate source of berberine and the plant has high antioxidant and antimicrobial properties owing to its berberine content.
草药的质量由其次生代谢产物决定，这些次生代谢产物可能会因生长、季节和海拔等因素而有所不同。目的：我们研究了从四个不同海拔地点采集的唐松草根的植物化学和生物活性变化。
采用高效薄层色谱法（HPTLC）测定从不同海拔采集的唐松草根不同提取物中的小檗碱含量。分别使用福林-西奥尔特试剂和氯化铝法测定总酚和黄酮含量。采用纸片扩散法研究提取物对微生物的敏感性，并采用肉汤稀释法估算最低抑菌浓度（MIC）。使用β-胡萝卜素漂白试验、山羊肝脂质过氧化试验、还原能力试验和DPPH自由基清除活性研究该植物的抗氧化能力。
小檗碱含量与海拔呈负相关；而唐松草的酚类和黄酮类含量在较高海拔处增加。所有唐松草提取物对白色念珠菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌均表现出中度至高活性。小檗碱含量高的提取物对白色念珠菌和金黄色葡萄球菌最有效，并且还表现出相对显著的抗脂质过氧化、β-胡萝卜素漂白和还原能力。酚类和黄酮类含量较高的唐松草提取物对DPPH自由基的清除效果更好。在所有进行的抗氧化和抗菌实验中，均以小檗碱作为标准。
低海拔地区的唐松草可作为小檗碱的替代来源进行开发，并且由于其小檗碱含量，该植物具有较高的抗氧化和抗菌特性。
