通过核磁共振波谱揭示富含硫酸岩藻糖的多糖的结构。

Unveiling the structure of sulfated fucose-rich polysaccharides via nuclear magnetic resonance spectroscopy.

机构信息

Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, P.O. Box 68041, Rio de Janeiro, RJ 21941-913, Brazil.

出版信息

Curr Opin Struct Biol. 2018 Jun;50:33-41. doi: 10.1016/j.sbi.2017.10.011. Epub 2017 Nov 5.

DOI:10.1016/j.sbi.2017.10.011

PMID:29102626

Abstract

Sulfated fucans from marine invertebrates are composed of regular repetitive fucose building-blocks with sulfation patterns differing in a species-specific manner. These polysaccharides can act as mediators of the acrosome reaction of sea-urchins or play a structural role in the body-wall of sea-cucumbers. Other fucose-rich polysaccharides found in the body-wall of sea-cucumbers are the fucosylated chondroitin sulfates composed of a vertebrate-like chondroitin sulfate decorated with species-specific fucose branches. Fine-tuning structural determinations of these polysaccharides have been accomplished since the 1980s almost exclusively via high-resolution NMR. In this review, we present an overview on NMR-based structural and conformational analyses of these sulfated fucose-rich polysaccharides. This constitutes an outstanding example of the potential of NMR in studying the chemical aspects of complex carbohydrates.

摘要

海洋无脊椎动物来源的硫酸化岩藻聚糖由结构重复的岩藻糖基组成，其硫酸化模式在物种特异性上存在差异。这些多糖可以作为海胆顶体反应的介质，或者在海参体壁中发挥结构作用。海参体壁中还存在其他富含岩藻糖的多糖，即硫酸软骨素岩藻聚糖，由类似脊椎动物的硫酸软骨素组成，上面装饰有特定于物种的岩藻糖支链。自 20 世纪 80 年代以来，几乎完全通过高分辨率 NMR 来实现对这些多糖结构的精细调控。在这篇综述中，我们介绍了基于 NMR 的这些富含硫酸化岩藻糖多糖的结构和构象分析。这是 NMR 在研究复杂碳水化合物化学方面潜力的一个杰出范例。