Hsa_circ_0000520，一种潜在的新型环状 RNA 生物标志物，与胃癌有关。
Hsa_circ_0000520, a potential new circular RNA biomarker, is involved in gastric carcinoma.
机构信息
Department of Oncology Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
出版信息
Cancer Biomark. 2018 Feb 6;21(2):299-306. doi: 10.3233/CBM-170379.
BACKGROUND
Circular RNAs (circRNAs) have been found playing important roles in regulating cancer progression. Human circRNA microarray was performed to screen for abnormally expressed circRNA in gastric cancer tissues. In this study, we are aimed to investigate the relationship between a new circular RNA named hsa_circ_0000520 and gastric cancer development.
METHODS
The hsa_circ_0000520 levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in gastric tissue, cell and plasma levels, respectively. Then, the association between the expression level of hsa_circ_0000520 and the clinicopathological features of patients with gastric cancer was further analyzed. Finally, a network of hsa_circ_0000520-miRNA-mRNA interactions was predicated.
RESULTS
In this study, hsa_circ_0000520 was first found to be significantly down-regulated in gastric cancer tissues, plasma and gastric cancer cell lines compared with control cases. Clinicopathological features showed that hsa_circ_0000520 level in GC tissues was negatively associated with TNM stage and in GC plasma linked with CEA expression. Finally, a total of 9 miRNAs and 9 candidate mRNA were predicted to have an interaction with hsa_circ_0000520.
CONCLUSIONS
We first identified that hsa_circ_0000520 was significantly down-regulated in gastric cancer. Our study indicated hsa_circ_0000520 might serve as a novel biomarker for gastric cancer and is involved in gastric carcinoma development.
背景
环状 RNA（circRNAs）已被发现在调控癌症进展中发挥重要作用。我们进行了人类环状 RNA 微阵列分析，以筛选胃癌组织中异常表达的环状 RNA。在本研究中，我们旨在研究一种名为 hsa_circ_0000520 的新环状 RNA 与胃癌发生发展的关系。
方法
分别通过定量逆转录聚合酶链反应（qRT-PCR）检测胃癌组织、细胞和血浆中的 hsa_circ_0000520 水平。然后，进一步分析 hsa_circ_0000520 表达水平与胃癌患者临床病理特征的关系。最后，预测了 hsa_circ_0000520-miRNA-mRNA 相互作用网络。
结果
本研究首次发现，与对照组相比，胃癌组织、血浆和胃癌细胞系中 hsa_circ_0000520 的表达水平显著下调。临床病理特征分析显示，GC 组织中 hsa_circ_0000520 水平与 TNM 分期呈负相关，GC 血浆中 hsa_circ_0000520 水平与 CEA 表达相关。最后，共预测到 9 个 miRNA 和 9 个候选 mRNA 与 hsa_circ_0000520 具有相互作用。
结论
我们首次发现 hsa_circ_0000520 在胃癌中显著下调。本研究表明，hsa_circ_0000520 可能作为胃癌的一种新型生物标志物，参与胃癌的发生发展。
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