Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Department of Oncology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
Cancer Biomark. 2020;27(2):139-145. doi: 10.3233/CBM-182029.
Circular RNAs (circRNA) play key regulatory roles in cancer progression. Human circRNA microarray was performed to screen for abnormally expressed circRNA in gastric cancer tissues. In this study, we found circRNA102958 was up-regulated in gastric cancer tissues and cell lines.
The hsa_circRNA_102958 levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in gastric tissue and cell. Then, the association between the expression level of hsa_circRNA_102958 and the clinicopathological features of patients with gastric cancer was further analyzed. Finally, a network of hsa_circRNA_102958-miRNA-mRNA interactions was predicated.
In this study, we analyzed 30 patients and found that hsa_circRNA_102958 was significantly overexpressed in gastric cancer tissues compared with paired adjacent normal tissues. Clinicopathological features showed that hsa_circRNA_102958 level in GC tissues was positively associated with TNM stage (p= 0.032). The area under the ROC curve was 0.74. Finally, a total of 5 miRNAs were predicted to have an interaction with hsa_circRNA_102958.
hsa_circRNA_102958 may be a potential novel and stable biomarker for the diagnosis of gastric carcinoma.
环状 RNA（circRNA）在癌症进展中发挥关键调节作用。进行了人类 circRNA 微阵列以筛选胃癌组织中异常表达的 circRNA。在本研究中，我们发现 circRNA102958 在胃癌组织和细胞中上调。
通过定量逆转录-聚合酶链反应（qRT-PCR）在胃癌组织和细胞中检测 hsa_circRNA_102958 水平。然后，进一步分析 hsa_circRNA_102958 的表达水平与胃癌患者临床病理特征之间的关系。最后，预测了 hsa_circRNA_102958-miRNA-mRNA 相互作用网络。
在本研究中，我们分析了 30 名患者，发现 hsa_circRNA_102958 在胃癌组织中明显过表达，与配对的相邻正常组织相比。临床病理特征显示，GC 组织中 hsa_circRNA_102958 水平与 TNM 分期呈正相关（p=0.032）。ROC 曲线下面积为 0.74。最后，总共预测了 5 个 miRNA 与 hsa_circRNA_102958 具有相互作用。
hsa_circRNA_102958 可能是胃癌诊断的一种潜在的新型稳定生物标志物。
