State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Carbohydr Polym. 2018 Jan 15;180:238-245. doi: 10.1016/j.carbpol.2017.10.026. Epub 2017 Oct 7.
The chain conformation, chemical characters and immunomodulatory activity of polysaccharide from Dendrobium devonianum (DDP) were investigated. Results showed that molecular weights, polydispersity index, radius of gyrations of DDP were 3.99×10 Da1.27, 74.1nm, respectively. By applying the polymer solution theory, the exponent (v) values of =kM was calculated as 0.38, which revealed that DDP existed as a globular shape in aqueous solution, and further confirmed by AFM analysis. Furthermore, the main monosaccharide compositions were Man and Glc with the ratio of 29.61:1.00. Indeed, the main glycosidic linkages were β-1,4-Manp, and substituted with acetyl groups at O-2 and O-3 position. Notably, DDP could promote the immune functions of macrophages including NO release and phagocytosis. Thus, DDP could be explored as a natural immune-stimulating agent in the health and functional food area as well as pharmaceutical industries.
研究了金钗石斛多糖（DDP）的链构象、化学性质和免疫调节活性。结果表明，DDP 的分子量、多分散指数和回转半径分别为 3.99×10 Da、1.27 和 74.1nm。通过应用聚合物溶液理论，计算出 =kM 中的指数（v）值为 0.38，表明 DDP 在水溶液中呈球形存在，并通过 AFM 分析进一步证实。此外，主要单糖组成为 Man 和 Glc，比例为 29.61:1.00。实际上，主要糖苷键为β-1,4-Manp，并在 O-2 和 O-3 位置被乙酰化取代。值得注意的是，DDP 可以促进巨噬细胞的免疫功能，包括 NO 释放和吞噬作用。因此，DDP 可以作为天然免疫刺激剂在健康和功能性食品领域以及制药行业中进行探索。
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