RNA-Seq-derived identification of differential transcription in the eggplant (Solanum melongena) following inoculation with bacterial wilt.

Eggplant (Solanum melongena) is a major vegetable crop worldwide. However, it is susceptible to bacterial wilt (BW) caused by Ralstonia solanacearum, which has become an important factor limiting eggplant yield and quality. The underlying mechanism of BW remains unknown. Here, RNA-sequencing was used to characterize the transcriptomes of resistant (R) and susceptible (S) strains before (R0, S0) and after (R1, S1) R. solanacearum inoculation. After the removal of low-quality sequences and assembly, 125,852 contigs, 122,508 transcripts, and 68,792 unigenes were identified, with 51,165 non-redundant unigenes annotated. Functional annotations were provided for 11,039 unigenes using four databases (NCBI Nr, Swissprot, KEGG and COG database). A total of 1137 and 9048 genes were found to be up- and down-regulated, respectively, in R0 relative to R1 samples, with 738 and 217 up- and down-regulated in S0 relative to R0 samples, 6087 and 5832 up- and down-regulated in S0 relative to S1 samples, and 4712 and 12,523 up- and down-regulated in S1 relative to R1 samples, respectively. In conclusion, our results provide useful insights into the potential mechanism of BW and are an important basis for further analysis.