College of Agriculture, Guangxi University, Nanning 530004, China.
Int J Mol Sci. 2021 Dec 10;22(24):13279. doi: 10.3390/ijms222413279.
L. (eggplant) bacterial wilt is a severe soil borne disease. Here, this study aimed to explore the regulation mechanism of eggplant bacterial wilt-resistance by transcriptomics with weighted gene co-expression analysis network (WGCNA). The different expression genes (DEGs) of roots and stems were divided into 21 modules. The module of interest (root: indianred4, stem: coral3) with the highest correlation with the target traits was selected to elucidate resistance genes and pathways. The selected module of roots and stems co-enriched the pathways of MAPK signalling pathway, plant pathogen interaction, and glutathione metabolism. Each top 30 hub genes of the roots and stems co-enriched a large number of receptor kinase genes. A total of 14 interesting resistance-related genes were selected and verified with quantitative polymerase chain reaction (qPCR). The qPCR results were consistent with those of WGCNA. The hub gene of (namely ) was further functionally verified; positively regulated the resistance of eggplant to bacterial wilt by qPCR and virus-induced gene silencing (VIGS). Our study provides a reference for the interaction between eggplants and bacterial wilt and the breeding of broad-spectrum and specific eggplant varieties that are bacterial wilt-resistant.
（茄子）青枯病是一种严重的土传病害。本研究旨在通过加权基因共表达网络分析（WGCNA）的转录组学来探索茄子青枯病抗性的调控机制。将根和茎的差异表达基因（DEGs）分为 21 个模块。选择与目标性状相关性最高的感兴趣模块（根：indianred4，茎：coral3），以阐明抗性基因和途径。选定的根和茎模块共同富集了 MAPK 信号通路、植物病原体相互作用和谷胱甘肽代谢途径。根和茎的前 30 个枢纽基因共同富集了大量的受体激酶基因。共选择了 14 个有趣的与抗性相关的基因，并通过定量聚合酶链反应（qPCR）进行了验证。qPCR 结果与 WGCNA 的结果一致。进一步对（即）的枢纽基因进行了功能验证；通过 qPCR 和病毒诱导基因沉默（VIGS）证实正向调控茄子对青枯病的抗性。本研究为茄子与青枯病的互作以及广谱和特异茄子品种的抗病性育种提供了参考。
