Department of Minimally Invasive Neurosurgery, Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China (mainland).
Heilongjiang Medical Science Institute, Harbin, Heilongjiang, China (mainland).
Med Sci Monit. 2017 Nov 6;23:5277-5282. doi: 10.12659/msm.904554.
BACKGROUND To explore the theoretical basis for protecting the brain from ischemic stroke with tetramethylpyrazine, we studied whether and how tetramethylpyrazine could protect neurons against the oxygen-glucose deprivation (OGD)-induced death and whether transient receptor potential cation channel, subfamily C, member 6 (TRPC6) was involved. MATERIAL AND METHODS Primary rat cortical neurons were cultured and an OGD model was established in the presence or absence of tetramethylpyrazine. Neuronal death was assessed by measuring the uptake of membrane-impermeable PI. Western blot analysis was used to determine the protein expressions of TRPC6 and caspase-3. The involvement of TRPC6 was tested via RNAi against TRPC6. RESULTS OGD-induced neuronal death was decreased by tetramethylpyrazine in a concentration-dependent manner. The expression of TRPC6 protein was decreased by OGD. Furthermore, downregulating TRPC6 by RNA interfering mimicked the effect of OGD in neuronal death. Tetramethylpyrazine attenuated OGD-induced TRPC6 downregulation in a tetramethylpyrazine concentration-dependent manner. However, these effects of tetramethylpyrazine on attenuating OGD-induced neuronal death were abolished by TRPC6 RNAi. CONCLUSIONS Tetramethylpyrazine can protect neurons from oxygen-glucose deprivation-induced death, possibly via TRPC6.
为了探索川芎嗪保护脑缺血性中风的理论基础，我们研究了川芎嗪是否以及如何保护神经元免受氧葡萄糖剥夺（OGD）诱导的死亡，以及瞬时受体电位阳离子通道亚家族 C 成员 6（TRPC6）是否参与其中。
原代大鼠皮质神经元培养，在存在或不存在川芎嗪的情况下建立 OGD 模型。通过膜不可渗透的 PI 摄取来评估神经元死亡。Western blot 分析用于确定 TRPC6 和 caspase-3 的蛋白表达。通过针对 TRPC6 的 RNAi 测试 TRPC6 的参与。
OGD 诱导的神经元死亡呈浓度依赖性被川芎嗪降低。TRPC6 蛋白的表达被 OGD 降低。此外，通过 RNA 干扰下调 TRPC6 模拟了 OGD 诱导的神经元死亡。川芎嗪以川芎嗪浓度依赖性方式减弱 OGD 诱导的 TRPC6 下调。然而，TRPC6 RNAi 消除了川芎嗪对 OGD 诱导的神经元死亡的这些作用。
川芎嗪可以保护神经元免受氧葡萄糖剥夺诱导的死亡，可能通过 TRPC6。
