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用于细胞成像的荧光探针和标记物。

Fluorescent Probes and Labels for Cellular Imaging.

作者信息

Fahrni Christoph J

出版信息

Chimia (Aarau). 2009 Nov;63(11):714-720. doi: 10.2533/chimia.2009.714.

DOI:10.2533/chimia.2009.714
PMID:29104302
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5667356/
Abstract

Metal-responsive fluorescent indicators are powerful tools for visualizing trace metals with subcellular resolution. By taking advantage of the diverse photophysical properties of organic fluorophores, metal ion-selective fluorescent indicators have been rationally designed and tailored towards cellular applications. This review summarizes challenges associated with the probe design and describes recent efforts in our research group in developing selective and sensitive reagents for the detection of zinc and copper in mammalian cells.

摘要

金属响应荧光指示剂是用于以亚细胞分辨率可视化痕量金属的强大工具。通过利用有机荧光团的多种光物理性质，已合理设计并定制了针对细胞应用的金属离子选择性荧光指示剂。本综述总结了与探针设计相关的挑战，并描述了我们研究小组最近在开发用于检测哺乳动物细胞中锌和铜的选择性和灵敏试剂方面所做的努力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/47137a8d2316/nihms876190f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/5afad83fbbfb/nihms876190f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/bb96612081d3/nihms876190f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/b0dd105cb218/nihms876190f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/e638e4da557b/nihms876190f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/71f788e01041/nihms876190f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/4005f1cd5d38/nihms876190f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/3812aaf896b4/nihms876190f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/dee6c4fa4d21/nihms876190f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/47137a8d2316/nihms876190f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/5afad83fbbfb/nihms876190f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/bb96612081d3/nihms876190f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/b0dd105cb218/nihms876190f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/e638e4da557b/nihms876190f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/71f788e01041/nihms876190f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/4005f1cd5d38/nihms876190f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/3812aaf896b4/nihms876190f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/dee6c4fa4d21/nihms876190f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41bb/5667356/47137a8d2316/nihms876190f9.jpg

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本文引用的文献

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In situ imaging of metals in cells and tissues.细胞和组织中金属的原位成像。
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