纳秒脉冲介质阻挡放电处理产生的反应化学作用导致了B16黑色素瘤小鼠模型中的肿瘤消除。
Reaction Chemistry Generated by Nanosecond Pulsed Dielectric Barrier Discharge Treatment is Responsible for the Tumor Eradication in the B16 Melanoma Mouse Model.
作者信息
Chernets Natalie, Kurpad Deepa S, Alexeev Vitali, Rodrigues Dario B, Freeman Theresa A
机构信息
Department of Orthopaedic Surgery, Thomas Jefferson, University, 1015 Walnut Street, Philadelphia, Pennsylvania 19107.
Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
出版信息
Plasma Process Polym. 2015 Dec;12(12):1400-1409. doi: 10.1002/ppap.201500140. Epub 2015 Oct 12.
Abstract
Melanoma is one of the most aggressive metastatic cancers with resistance to radiation and most chemotherapy agents. This study highlights an alternative treatment for melanoma based on nanosecond pulsed dielectric barrier discharge (nsP DBD). We show that a single nsP DBD treatment, directly applied to a 5 mm orthotopic mouse melanoma tumor, completely eradicates it 66% ( = 6; ≤ 0.05) of the time. It was determined that reactive oxygen and nitrogen species produced by nsP DBD are the main cause of tumor eradication, while nsP electric field and heat generated by the discharge are not sufficient to kill the tumor. However, we do not discount that potential synergy between each plasma generated component (temperature, electric field and reactive species) can enhance the killing efficacy.
摘要
黑色素瘤是最具侵袭性的转移性癌症之一，对放疗和大多数化疗药物具有抗性。本研究重点介绍了一种基于纳秒脉冲介质阻挡放电（nsP DBD）的黑色素瘤替代治疗方法。我们发现，直接对5毫米原位小鼠黑色素瘤肿瘤进行单次nsP DBD治疗，有66%（n = 6；P≤0.05）的概率能将其完全根除。研究确定，nsP DBD产生的活性氧和氮物种是肿瘤根除的主要原因，而放电产生的nsP电场和热量不足以杀死肿瘤。然而，我们并不排除各等离子体产生成分（温度、电场和活性物种）之间的潜在协同作用可提高杀伤效果。
相似文献
1
Reaction Chemistry Generated by Nanosecond Pulsed Dielectric Barrier Discharge Treatment is Responsible for the Tumor Eradication in the B16 Melanoma Mouse Model.纳秒脉冲介质阻挡放电处理产生的反应化学作用导致了B16黑色素瘤小鼠模型中的肿瘤消除。
Plasma Process Polym. 2015 Dec;12(12):1400-1409. doi: 10.1002/ppap.201500140. Epub 2015 Oct 12.
2
Uniform and non-uniform modes of nanosecond-pulsed dielectric barrier discharge in atmospheric air: fast imaging and spectroscopic measurements of electric field.大气空气中纳秒脉冲介质阻挡放电的均匀和非均匀模式：电场的快速成像和光谱测量
J Phys D Appl Phys. 2014 Jun 25;47(25):252003. doi: 10.1088/0022-3727/47/25/252003.
3
Nanosecond pulsed uniform dielectric barrier discharge in atmospheric air: A brief spectroscopic analysis.纳秒脉冲突发均匀介质阻挡放电的大气中：简要光谱分析。
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 15;207:294-300. doi: 10.1016/j.saa.2018.09.004. Epub 2018 Sep 4.
4
Numerical Investigation on the Effects of Dielectric Barrier on a Nanosecond Pulsed Surface Dielectric Barrier Discharge.关于介质阻挡对纳秒脉冲表面介质阻挡放电影响的数值研究。
Molecules. 2019 Oct 31;24(21):3933. doi: 10.3390/molecules24213933.
5
Non-Equilibrium Dielectric Barrier Discharge Treatment of Mesenchymal Stem Cells: Charges and Reactive Oxygen Species Play the Major Role in Cell Death.间充质干细胞的非平衡介质阻挡放电处理：电荷和活性氧在细胞死亡中起主要作用。
Plasma Process Polym. 2015 Oct;12(10):1117-1127. doi: 10.1002/ppap.201400232. Epub 2015 Apr 14.
6
Chemical modification of extracellular matrix by cold atmospheric plasma-generated reactive species affects chondrogenesis and bone formation.冷大气等离子体产生的活性物质对细胞外基质的化学修饰影响软骨形成和骨形成。
J Tissue Eng Regen Med. 2016 Sep;10(9):772-82. doi: 10.1002/term.2224. Epub 2016 Aug 11.
7
Discharge Regimes Transition and Characteristics Evolution of Nanosecond Pulsed Dielectric Barrier Discharge.纳秒脉冲介质阻挡放电的放电模式转变与特性演化
Nanomaterials (Basel). 2019 Sep 26;9(10):1381. doi: 10.3390/nano9101381.
8
Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress.纳秒脉冲介质阻挡放电等离子体产生的活性氧通过细胞内氧化应激触发A549肺癌细胞的免疫原性细胞死亡。
Int J Mol Sci. 2017 May 3;18(5):966. doi: 10.3390/ijms18050966.
9
Air disinfection by nanosecond pulsed DBD plasma.纳秒脉冲介质阻挡放电等离子体进行空气消毒
J Hazard Mater. 2024 Jul 5;472:134487. doi: 10.1016/j.jhazmat.2024.134487. Epub 2024 May 3.
10
A Novel Dielectric Barrier Discharge (DBD) Reactor with Streamer and Glow Corona Discharge for Improved Ozone Generation at Atmospheric Pressure.一种新型介质阻挡放电（DBD）反应器，具有流光和辉光放电，用于在大气压下提高臭氧生成量。
Micromachines (Basel). 2021 Oct 21;12(11):1287. doi: 10.3390/mi12111287.
引用本文的文献
1
Evaluating the Healing Potential of J-Plasma Scalpel-Created Surgical Incisions in Porcine and Rat Models.评估J型等离子手术刀在猪和大鼠模型中所造成手术切口的愈合潜力。
Biomedicines. 2024 Jan 25;12(2):277. doi: 10.3390/biomedicines12020277.
2
No-ozone cold plasma induces apoptosis in human neuroblastoma cell line via increased intracellular reactive oxygen species (ROS).无臭氧冷等离子体通过增加细胞内活性氧物种 (ROS) 诱导人神经母细胞瘤细胞系凋亡。
BMC Complement Med Ther. 2024 Jan 20;24(1):46. doi: 10.1186/s12906-023-04313-0.
3
Synergistic Inhibitory Effect of Berberine and Low-Temperature Plasma on Non-Small-Cell Lung Cancer Cells via PI3K-AKT-Driven Signaling Axis.小檗碱与低温等离子体通过 PI3K-AKT 信号轴对非小细胞肺癌细胞的协同抑制作用。
Molecules. 2023 Nov 27;28(23):7797. doi: 10.3390/molecules28237797.
4
The Development of Nonthermal Plasma and Tirapazamine as a Novel Combination Therapy to Treat Melanoma In Situ.非热等离子体与替拉扎明联合治疗原位黑素瘤的新探索。
Cells. 2023 Aug 21;12(16):2113. doi: 10.3390/cells12162113.
5
Anticancer Effect of Cold Atmospheric Plasma in Syngeneic Mouse Models of Melanoma and Colon Cancer.冷等离体子在黑色素瘤和结肠癌同基因小鼠模型中的抗癌作用。
Molecules. 2023 May 18;28(10):4171. doi: 10.3390/molecules28104171.
6
The effect of local non-thermal plasma therapy on the cancer-immunity cycle in a melanoma mouse model.局部非热等离子体疗法对黑色素瘤小鼠模型中癌症免疫循环的影响。
Bioeng Transl Med. 2022 Apr 21;7(3):e10314. doi: 10.1002/btm2.10314. eCollection 2022 Sep.
7
Low Temperature Plasma Suppresses Lung Cancer Cells Growth via VEGF/VEGFR2/RAS/ERK Axis.低温等离子体通过 VEGF/VEGFR2/RAS/ERK 轴抑制肺癌细胞生长。
Molecules. 2022 Sep 13;27(18):5934. doi: 10.3390/molecules27185934.
8
Preclinical Cold Atmospheric Plasma Cancer Treatment.临床前冷大气等离子体癌症治疗
Cancers (Basel). 2022 Jul 16;14(14):3461. doi: 10.3390/cancers14143461.
9
Cold Atmospheric Plasma Ameliorates Skin Diseases Involving Reactive Oxygen/Nitrogen Species-Mediated Functions.冷等离体子体减轻涉及活性氧/氮物种介导功能的皮肤疾病。
Front Immunol. 2022 May 26;13:868386. doi: 10.3389/fimmu.2022.868386. eCollection 2022.
10
Oxidative Stress Differentially Influences the Survival and Metabolism of Cells in the Melanoma Microenvironment.氧化应激对黑色素瘤微环境中细胞的存活和代谢有不同的影响。
Cells. 2022 Mar 8;11(6):930. doi: 10.3390/cells11060930.
本文引用的文献
1
Non-Equilibrium Dielectric Barrier Discharge Treatment of Mesenchymal Stem Cells: Charges and Reactive Oxygen Species Play the Major Role in Cell Death.间充质干细胞的非平衡介质阻挡放电处理：电荷和活性氧在细胞死亡中起主要作用。
Plasma Process Polym. 2015 Oct;12(10):1117-1127. doi: 10.1002/ppap.201400232. Epub 2015 Apr 14.
2
Differential Effects of Cold Atmospheric Plasma in the Treatment of Malignant Glioma.冷大气等离子体治疗恶性胶质瘤的差异效应
PLoS One. 2015 Jun 17;10(6):e0126313. doi: 10.1371/journal.pone.0126313. eCollection 2015.
3
In Situ OH Generation from O2- and H2O2 Plays a Critical Role in Plasma-Induced Cell Death.由超氧阴离子和过氧化氢原位生成羟基自由基在等离子体诱导的细胞死亡中起关键作用。
PLoS One. 2015 Jun 5;10(6):e0128205. doi: 10.1371/journal.pone.0128205. eCollection 2015.
4
Differential Epigenetic Effects of Atmospheric Cold Plasma on MCF-7 and MDA-MB-231 Breast Cancer Cells.大气冷等离子体对MCF-7和MDA-MB-231乳腺癌细胞的差异表观遗传效应
PLoS One. 2015 Jun 4;10(6):e0129931. doi: 10.1371/journal.pone.0129931. eCollection 2015.
5
Identification of the biologically active liquid chemistry induced by a nonthermal atmospheric pressure plasma jet.非热大气压等离子体射流诱导产生的生物活性液体化学物质的鉴定
Biointerphases. 2015 Jun 6;10(2):029518. doi: 10.1116/1.4919710.
6
On the design and characterization of a new cold atmospheric pressure plasma jet and its applications on cancer cells treatment.新型冷大气压等离子体射流的设计、表征及其在癌细胞治疗中的应用
Biointerphases. 2015 Jun 23;10(2):029510. doi: 10.1116/1.4918806.
7
Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells.低温等离子体处理会导致原发性前列腺上皮细胞中的DNA损伤，进而引发坏死性细胞死亡。
Br J Cancer. 2015 Apr 28;112(9):1536-45. doi: 10.1038/bjc.2015.113. Epub 2015 Apr 2.
8
Use of cold atmospheric plasma in the treatment of cancer.冷大气等离子体在癌症治疗中的应用。
Biointerphases. 2015 Jun 19;10(2):029403. doi: 10.1116/1.4915264.
9
Responses of solid tumor cells in DMEM to reactive oxygen species generated by non-thermal plasma and chemically induced ROS systems.在DMEM中实体瘤细胞对非热等离子体产生的活性氧以及化学诱导的ROS系统的反应。
Sci Rep. 2015 Feb 26;5:8587. doi: 10.1038/srep08587.
10
Effects of atmospheric pressure plasmas on isolated and cellular DNA-a review.大气压等离子体对分离DNA和细胞DNA的影响——综述
Int J Mol Sci. 2015 Jan 29;16(2):2971-3016. doi: 10.3390/ijms16022971.
Zotero 插件