Melnicoff M J, Horan P K, Morahan P S
Department of Immunology, Smith Kline & French Laboratories, King of Prussia, Pennsylvania 19406.
Cell Immunol. 1989 Jan;118(1):178-91. doi: 10.1016/0008-8749(89)90367-5.
The kinetics of macrophage (M phi) recruitment to the peritoneum following the induction of acute inflammation by thioglycollate broth (TG) was evaluated after prelabeling resident M phi with the fluorescent cell tracking dye, PKH-1. Most of the PKH-1-labeled resident M phi disappeared from the recoverable peritoneal cell population within the first hour after injection of TG. This disappearance coincided with the inflammatory influx of neutrophils (PMNs) and was sustained for at least 5 days after administration of TG, although the PMN number had returned to resident levels by this time. PKH-1-labeled peritoneal M phi were observed again in most animals at 7 days after injection of TG. The number of labeled M phi recovered at 7 days was approximately twice the number of resident peritoneal M phi in control animals which did not receive the TG broth. These additional M phi may include progeny of either the resident M phi or other local M phi precursors, such as omental M phi, which were labeled by the PKH-1 injection.
在用荧光细胞追踪染料PKH-1对驻留巨噬细胞（M phi）进行预标记后，评估了用巯基乙酸盐肉汤（TG）诱导急性炎症后巨噬细胞向腹膜募集的动力学。注射TG后的第一小时内，大多数PKH-1标记的驻留M phi从可回收的腹膜细胞群体中消失。这种消失与中性粒细胞（PMN）的炎性流入同时发生，并且在给予TG后至少持续5天，尽管此时PMN数量已恢复到驻留水平。在注射TG后7天，大多数动物中再次观察到PKH-1标记的腹膜M phi。在7天时回收的标记M phi数量约为未接受TG肉汤的对照动物中驻留腹膜M phi数量的两倍。这些额外的M phi可能包括驻留M phi或其他局部M phi前体（如网膜M phi）的后代，它们通过PKH-1注射被标记。
