Collet-Cassart D, Limet J N, Van Krieken L, De Hertogh R
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Clin Chem. 1989 Jan;35(1):141-3.
In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.
在这种用于人胎盘催乳素的乳胶免疫测定法中，微量滴定板用作反应容器，并测量405nm处的吸光度以定量反应。这个30分钟的测定仅需要一次血清稀释和两个移液步骤。校准曲线范围为0.5至15mg/L。批内测定的变异系数范围为4.2%至7.0%，批间测定的变异系数范围为7.0%至11.2%。当该方法与商业放射免疫测定法比较时，84份血清的相关系数为0.949。
