Samuel G, Sivaprasad N, Shah K B, Mani R S
Clin Chem. 1983 Jan;29(1):168-70.
We report a solid-phase radioimmunoassay for human placental lactogen, in which antibody-coated polystyrene tubes and polyurethane sponges are used. Incubation time and temperature, ionic strength of buffer, and nonspecific adsorption have been evaluated for optimum conditions required for routine clinical assays. The validity of the assay technique has been ascertained by its specificity, reproducibility, and recovery. The sensitivity of this method is 0.4 ng of placental lactogen per assay tube.
我们报告了一种用于人胎盘催乳素的固相放射免疫测定法，该方法使用了包被抗体的聚苯乙烯管和聚氨酯海绵。我们评估了孵育时间和温度、缓冲液的离子强度以及非特异性吸附，以确定常规临床检测所需的最佳条件。该检测技术的有效性已通过其特异性、可重复性和回收率得到证实。此方法的灵敏度为每个检测管0.4纳克胎盘催乳素。
