Sadaf Sana, Emad Shaista, Siddiqui Naveed Ali, Ghafoor Seemeen, Yousuf Sarwat, Jabeen Bushra, Haider Saida, Perveen Tahira
Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan.
Neurochemistry and Biochemical Neuropharmacology Research Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan / Jinnah College of Pharmacy, Jinnah Medical and Dental College, Karachi, Pakistan.
Pak J Pharm Sci. 2017 Sep;30(5(Supplementary)):2039-2046.
Stress is a vulnerable state to cellular homeostasis which leads to oxidative damage via free radical generation. The acute stress induces alteration in antioxidant enzyme activities to an extent which produce oxidative stress and causes certain pathological conditions. The use of Nigella sativa L. oil (NSO) in folk medicine has increased throughout the world for the prevention or treatment of various ailments because of potent antioxidant properties. In the present study, potential therapeutic effects of NSO on memory in both unrestrained and 2h restrained rats were observed. Shortterm memory (STM) and long-term memory (LTM) were assessed by elevated plus maze (EPM) and Morris water maze (MWM) respectively. The present study also demonstrated the effect of NSO on lipid peroxidation (LPO) and activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) along with the activity of acetyl cholinesterase (AChE). The results obtained from the present study showed that 2h restraint stress significantly enhanced both short-term memory (p<0.01) and long-term memory (p<0.05) in rats. Pretreatment with NSO at a dose of 0.2ml/kg/day also significantly improved STM (p<0.05) in restrained rats and LTM (p<0.01) in unrestrained rats. This study also showed significantly decreased (p<0.01) LPO and significantly increased (p<0.01) endogenous antioxidant enzymes activity in NSO treated restrained rats. Similarly significant decreased (p<0.01) AChE activity was also observed in NSO treated unrestrained and 2h restrained rats. Therefore, current findings suggested that repeated administration of NSO may exert memory enhancing effects against restrained stress and it can be used for therapeutic purpose because of having fewer side effects.
应激是细胞内环境稳定的一种脆弱状态，可通过自由基生成导致氧化损伤。急性应激会使抗氧化酶活性发生改变，达到产生氧化应激并引发某些病理状况的程度。由于具有强大的抗氧化特性，黑种草籽油（NSO）在世界各地民间医学中用于预防或治疗各种疾病的应用日益增加。在本研究中，观察了NSO对自由活动和束缚2小时大鼠记忆的潜在治疗作用。分别通过高架十字迷宫（EPM）和莫里斯水迷宫（MWM）评估短期记忆（STM）和长期记忆（LTM）。本研究还证明了NSO对脂质过氧化（LPO）、抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）活性以及乙酰胆碱酯酶（AChE）活性的影响。本研究结果表明，2小时束缚应激显著增强了大鼠的短期记忆（p<0.01）和长期记忆（p<0.05）。以0.2ml/kg/天的剂量对束缚大鼠进行NSO预处理也显著改善了STM（p<0.05），对自由活动大鼠的LTM有显著改善（p<0.01）。该研究还表明，NSO处理的束缚大鼠中LPO显著降低（p<0.叭），内源性抗氧化酶活性显著增加（p<0.01）。同样，在NSO处理的自由活动和束缚2小时大鼠中也观察到AChE活性显著降低（p<0.01）。因此，目前的研究结果表明，重复给予NSO可能对束缚应激具有记忆增强作用，并且由于副作用较少，可用于治疗目的。
