Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, 211007, India.
Department of Pharmaceutical Science, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, India.
Biomed Pharmacother. 2018 Oct;106:575-583. doi: 10.1016/j.biopha.2018.06.160. Epub 2018 Jul 11.
Citrous lemon (Rutaceae) an Indian folk medicine has been used for the treatment of various pathological diseases viz., diabetes, cardiovascular, inflammation, hepatobiliary dysfunction and neurodegenerative disorder. Can lemon oil altered the memory of unstressed and stressed mice, a basic question for which the present work was put on trial. The present investigation was intended to assess the impact of Lemon oil on memory of unstressed and Stressed Swiss young Albino mice. Lemon oil (50 and 100 mg/kg o.r.) and donepezil (10 mg/kg) were guided for three weeks to different groups of stressed and unstressed mice. The nootropic movement was assessed utilizing elevated plus maze and Hebbs Williams Maze. Cerebrum acetylcholinesterase (AChE), plasmacorticosterone, decreased glutathione, lipid per oxidation alongside superoxide dismutase and catalase was surveyed as marker for disease. Histopathology was performed for estimation of drug effects. Acute immobilized stress was induce, lemon oil (100 mg/kg) and donepezil together indicated memory enhancing movement both in stressed and unstressed mice. Lemon oil significantly (p < 0.001) altered and lowered brain AChE activity both in stressed and unstressed mice. Scopolamine induced amnesia was also significantly altered and reversed both in stressed and unstressed mice by lemon oil at a dose of 50 and 100 mg/kg. Lemon oil (50 and 100 mg/kg) was further able to control the corticosterone level in plasma for stressed mice. Lemon oil significantly (p < 0.001) elevated the level of catalase, superoxide dismutase and reduced glutathione levels both in stressed and unstressed animals with respect to controlled group along with TBARS both in stressed and unstressed compared with control group. Hence it can be concluded that memory enhancing activity might be related to reduction in AChE and TBARS activity and by elevated GSH, SOD and catalase through decrease in raised plasma corticosterone levels.
柠檬（芸香科）是印度民间医学中用于治疗各种病理疾病的药物，如糖尿病、心血管疾病、炎症、肝胆功能障碍和神经退行性疾病。柠檬油是否能改变未应激和应激小鼠的记忆，这是一个基本问题，本研究旨在探讨这一问题。本研究旨在评估柠檬油对未应激和应激瑞士年轻白化病小鼠记忆的影响。柠檬油（50 和 100mg/kg 口服）和多奈哌齐（10mg/kg）分别用于三组应激和未应激小鼠，持续 3 周。使用高架十字迷宫和赫布氏威廉斯迷宫评估神经认知作用。脑乙酰胆碱酯酶（AChE）、血浆皮质酮、还原型谷胱甘肽、脂质过氧化以及超氧化物歧化酶和过氧化氢酶作为疾病标志物进行检测。进行组织病理学检查以评估药物作用。急性束缚应激诱导后，柠檬油（100mg/kg）和多奈哌齐联合使用可增强应激和未应激小鼠的记忆作用。柠檬油显著（p<0.001）改变并降低应激和未应激小鼠大脑中的 AChE 活性。柠檬油（50 和 100mg/kg）还能显著改变和逆转应激和未应激小鼠的东莨菪碱诱导的记忆障碍。柠檬油（50 和 100mg/kg）还能进一步控制应激小鼠血浆中的皮质酮水平。柠檬油显著（p<0.001）提高了应激和未应激动物的过氧化氢酶、超氧化物歧化酶和还原型谷胱甘肽水平，与对照组相比，应激和未应激组的 TBARS 水平也有所升高。因此，可以得出结论，增强记忆的作用可能与降低 AChE 和 TBARS 活性以及通过提高 GSH、SOD 和过氧化氢酶活性、降低升高的血浆皮质酮水平有关。
