Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan.
Biomed Pharmacother. 2018 Jan;97:765-770. doi: 10.1016/j.biopha.2017.10.165. Epub 2017 Nov 6.
Calcitonin (CTN), a calcium regulatory hormone, promotes calcium diuresis from the kidney and suppresses bone resorption. The objective of this study was to evaluate whether the topical and intermittent application of CTN inhibits alveolar bone resorption using ligature-induced experimental periodontitis in rats. Experimental periodontitis was induced by placing a nylon ligature around maxillary molars of 8-week-old male Wistar rats for 20 days. Thirty-two rats were divided into four groups: basal sham control group, periodontitis group, periodontitis plus 0.2 U CTN (low dose), and periodontitis plus 1.0 U CTN (high dose) group. To investigate the effects of CTN on alveolar bone resorption, CTN was topically injected into the palatal gingivae every 2 days after ligature removal (day 0). Micro-computed tomography (CT) analysis was performed for linear parameter assessment of alveolar bone on day 5 and day 14. Periodontal tissues were examined histo-pathologically to assess the differences among the study groups. Micro-CT images showed that alveolar bone resorption was induced statistically around the molar of ligatured rats on day 5 and day 14. The amount of bone resorption was more severe on day 14 than that on day 5. On day 5, only high-dose CTN treatment significantly suppressed bone resorption. In addition, both doses of CTN significantly suppressed bone resorption on day 14. Histological examination clarified that there were fewer TRAP-positive cells in the CTN treatment groups than in the periodontitis group on day 5. Local administration of CTN decreased alveolar bone resorption by regulating osteoclast activation in rats with periodontitis.
降钙素（CTN）是一种钙调节激素，可促进肾脏排钙，并抑制骨吸收。本研究旨在评估 CTN 的局部和间歇性应用是否通过结扎诱导的牙周炎大鼠模型抑制牙槽骨吸收。实验性牙周炎通过在 8 周龄雄性 Wistar 大鼠上颌磨牙周围放置尼龙结扎线 20 天来诱导。32 只大鼠分为 4 组：基础假手术对照组、牙周炎组、牙周炎加 0.2 U CTN（低剂量）组和牙周炎加 1.0 U CTN（高剂量）组。为了研究 CTN 对牙槽骨吸收的影响，在结扎去除后（第 0 天）每 2 天将 CTN 局部注射到腭牙龈中。在第 5 天和第 14 天进行微计算机断层扫描（CT）分析，以评估牙槽骨的线性参数。对牙周组织进行组织病理学检查，以评估各组之间的差异。微 CT 图像显示，第 5 天和第 14 天结扎大鼠磨牙周围的牙槽骨发生了统计学上的骨吸收。第 14 天的骨吸收量比第 5 天更严重。第 5 天，仅高剂量 CTN 治疗可显著抑制骨吸收。此外，两种剂量的 CTN 均可显著抑制第 14 天的骨吸收。组织学检查表明，在第 5 天，CTN 治疗组的 TRAP 阳性细胞数量少于牙周炎组。局部给予 CTN 可通过调节破骨细胞的激活来减少牙周炎大鼠的牙槽骨吸收。
