Seyedsadjadi Neda, Berg Jade, Bilgin Ayse A, Tung Chin, Grant Ross
School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia.
Australasian Research Institute, Sydney Adventist Hospital, Sydney, New South Wales, Australia.
PLoS One. 2017 Nov 6;12(11):e0187713. doi: 10.1371/journal.pone.0187713. eCollection 2017.
Oxidative stress has been closely linked to the progressive cell damage associated with emerging non-communicable disease (NCDs). Early detection of these biochemical abnormalities before irreversible cell damage occurs may therefore be useful in identifying disease risk at an individual level. In order to test this hypothesis, this study assessed the relationship between a simple measure of redox status and lifestyle risk factors for NCDs, and the population-based risk score of Framingham. In a cross-sectional study design, 100 apparently healthy middle-aged males (n = 48) and females (n = 52) were asked to complete a comprehensive lifestyle assessment questionnaire, followed by body fat percentage and blood pressure measurements, and blood collection. The ratio of plasma total antioxidant capacity to hydroperoxide (TAC/HPX) was used as an index of redox balance. One-way ANOVA and multiple linear regression analysis were performed to analyse the association between TAC/HPX, lifestyle components and other plasma biomarkers. The TAC/HPX ratio was higher in males compared to females (t96 = 2.34, P = 0.021). TAC/HPX was also lower in participants with poor sleep quality (t93 = 2.39, P = 0.019), with high sleep apnoea risk (t62.2 = 3.32, P = 0.002), with high caffeine (F(2, 93) = 3.97, P = 0.022) and red meat intake (F(2, 93) = 5.55, P = 0.005). These associations were independent of gender. Furthermore, the TAC/HPX ratio decreased with increasing body fat percentage (F(2, 95) = 4.74, P = 0.011) and depression score (t94 = 2.38, P = 0.019), though these associations were dependent on gender. Importantly, a negative association was observed between TAC/HPX levels and the Framingham risk score in both males (r(45) = -0.39, P = 0.008) and females (r(50) = -0.33, P = 0.019) that was independent of other Framingham risk score components. Findings from this study suggests that a relatively simple measure of redox balance such as the TAC/HPX ratio may be a sensitive indicator of redox stress, and may therefore serve as a useful biomarker for assessing an individual's specific NCD risk linked to unhealthy lifestyle practices.
氧化应激与新兴的非传染性疾病（NCDs）相关的进行性细胞损伤密切相关。因此，在不可逆转的细胞损伤发生之前早期检测这些生化异常，可能有助于在个体层面识别疾病风险。为了验证这一假设，本研究评估了氧化还原状态的简单测量指标与非传染性疾病的生活方式风险因素以及弗雷明汉姆人群风险评分之间的关系。在一项横断面研究设计中，100名表面健康的中年男性（n = 48）和女性（n = 52）被要求完成一份全面的生活方式评估问卷，随后测量体脂百分比、血压并采集血液。血浆总抗氧化能力与氢过氧化物的比值（TAC/HPX）被用作氧化还原平衡的指标。进行单因素方差分析和多元线性回归分析，以分析TAC/HPX、生活方式因素和其他血浆生物标志物之间的关联。男性的TAC/HPX比值高于女性（t96 = 2.34，P = 0.021）。睡眠质量差的参与者（t93 = 2.39，P = 0.019）、睡眠呼吸暂停风险高的参与者（t62.2 = 3.32，P = 0.002）、咖啡因摄入量高的参与者（F(2, 93) = 3.97，P = 0.022）和红肉摄入量高的参与者（F(2, 93) = 5.55，P = 0.005）的TAC/HPX也较低。这些关联与性别无关。此外，TAC/HPX比值随着体脂百分比的增加（F(2, 95) = 4.74，P = 0.011）和抑郁评分的增加（t94 = 2.38，P = 0.019）而降低，尽管这些关联依赖于性别。重要的是，在男性（r(45) = -0.39，P = 0.008）和女性（r(50) = -0.33，P = 0.019）中均观察到TAC/HPX水平与弗雷明汉姆风险评分之间存在负相关，且该相关性独立于其他弗雷明汉姆风险评分成分。本研究结果表明，诸如TAC/HPX比值这样相对简单的氧化还原平衡测量指标可能是氧化应激的敏感指标，因此可能作为评估个体与不健康生活方式相关的特定非传染性疾病风险的有用生物标志物。
