Lewis Nathan A, Newell John, Burden Richard, Howatson Glyn, Pedlar Charles R
English Institute of Sport, Athletes Training Village, University of Bath, Bath, United Kingdom.
School of Sport, Health and Applied Science, St Mary's University, Twickenham, London, United Kingdom.
PLoS One. 2016 Mar 1;11(3):e0149927. doi: 10.1371/journal.pone.0149927. eCollection 2016.
The longitudinal monitoring of oxidative stress (OS) in athletes may enable the identification of fatigued states and underperformance. The application of OS biomarker monitoring programs in sport are hindered by reliability and repeatability of in-the-field testing tools, the turnaround of results, and the understanding of biological variation (BV). Knowledge of BV and critical difference values (CDV) may assist with data interpretation in the individual athlete.
We aimed firstly to assess the repeatability of the clinical point of care redox test, Free Oxygen Radical Test (FORT) and the Free Oxygen Radical Defence (FORD) in trained participants and elite athletes and secondly to calculate the analytical, BV, CDV and index of individuality (II) for FORT, FORD, red blood cell glutathione, lutein, α and γ-tocopherol. Part 1: Fifteen elite athletes were sampled in duplicate for calculation of the repeatability of the FORT and FORD tests. Part 2: Twelve well-trained athletes had venous samples drawn every 2 hours from 0800 to 1800 for calculation of BV, CDV, II for FORT, FORD, RBC GSH, lutein, α-tocopherol and γ-tocopherol.
Repeatability of the FORT and FORD assay was 3.9% and 3.7% respectively. Biomarker CDV ranged from 12.8% to 37%, with a circadian effect for FORT, α-tocopherol and γ-tocopherol (p<0.01), with all biomarker indices of individuality < 0.8 arbitrary units.
We report that the use of the novel redox test in athletes is practical, and the generation of BV and CDV for biomarkers of OS enhances the interpretation of physiologically meaningful changes in individuals above the use of clinical reference ranges alone.
对运动员氧化应激（OS）进行纵向监测，可能有助于识别疲劳状态和运动表现不佳的情况。运动中OS生物标志物监测项目的应用受到现场测试工具的可靠性和可重复性、结果反馈周期以及对生物变异（BV）的理解等因素的阻碍。了解BV和临界差异值（CDV）可能有助于对个体运动员的数据进行解读。
我们的目标首先是评估临床即时氧化还原测试、游离氧自由基测试（FORT）和游离氧自由基防御（FORD）在训练有素的参与者和精英运动员中的可重复性，其次是计算FORT、FORD、红细胞谷胱甘肽、叶黄素、α和γ生育酚的分析变异、BV、CDV和个体性指数（II）。第1部分：对15名精英运动员进行重复采样，以计算FORT和FORD测试的可重复性。第2部分：12名训练有素的运动员在08:00至18:00每2小时采集一次静脉血样，以计算FORT、FORD、红细胞谷胱甘肽（RBC GSH）、叶黄素、α生育酚和γ生育酚的BV、CDV、II。
FORT和FORD检测的可重复性分别为3.9%和3.7%。生物标志物CDV范围为12.8%至37%，FORT、α生育酚和γ生育酚存在昼夜效应（p<0.01），所有生物标志物个体性指数<0.8任意单位。
我们报告称，在运动员中使用新型氧化还原测试是可行的，OS生物标志物的BV和CDV的生成增强了对个体生理上有意义变化的解读，这比仅使用临床参考范围更有意义。
