Yasir Muhammad
King Abdulaziz University, King Fahd Medical Research Center, Special Infectious Agents Unit, Jeddah, Saudi Arabia.
Braz J Microbiol. 2018 Apr-Jun;49(2):248-257. doi: 10.1016/j.bjm.2017.08.005. Epub 2017 Oct 18.
In this study for the first-time microbial communities in the caves located in the mountain range of Hindu Kush were evaluated. The samples were analyzed using culture-independent (16S rRNA gene amplicon sequencing) and culture-dependent methods. The amplicon sequencing results revealed a broad taxonomic diversity, including 21 phyla and 20 candidate phyla. Proteobacteria were dominant in both caves, followed by Bacteroidetes, Actinobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, and the archaeal phylum Euryarchaeota. Representative operational taxonomic units from Koat Maqbari Ghaar and Smasse-Rawo Ghaar were grouped into 235 and 445 different genera, respectively. Comparative analysis of the cultured bacterial isolates revealed distinct bacterial taxonomic profiles in the studied caves dominated by Proteobacteria in Koat Maqbari Ghaar and Firmicutes in Smasse-Rawo Ghaar. Majority of those isolates were associated with the genera Pseudomonas and Bacillus. Thirty strains among the identified isolates from both caves showed antimicrobial activity. Overall, the present study gave insight into the great bacterial taxonomic diversity and antimicrobial potential of the isolates from the previously uncharacterized caves located in the world's highest mountains range in the Indian sub-continent.
在本研究中，首次对兴都库什山脉洞穴中的微生物群落进行了评估。使用非培养方法（16S rRNA基因扩增子测序）和培养方法对样本进行了分析。扩增子测序结果显示出广泛的分类多样性，包括21个门和20个候选门。变形菌门在两个洞穴中均占主导地位，其次是拟杆菌门、放线菌门、厚壁菌门、疣微菌门、浮霉菌门以及古菌门广古菌门。来自科特·马克巴里·加尔洞穴和斯马斯-拉沃·加尔洞穴的代表性操作分类单元分别被归入235个和445个不同的属。对培养的细菌分离株进行的比较分析显示，在所研究的洞穴中，细菌分类谱不同，科特·马克巴里·加尔洞穴以变形菌门为主，斯马斯-拉沃·加尔洞穴以厚壁菌门为主。这些分离株中的大多数与假单胞菌属和芽孢杆菌属有关。从两个洞穴中鉴定出的分离株中有30株具有抗菌活性。总体而言，本研究深入了解了来自印度次大陆世界最高山脉中以前未被描述的洞穴的分离株的巨大细菌分类多样性和抗菌潜力。
