Canalis E, Centrella M, Burch W, McCarthy T L
Department of Medicine (Endocrine Section), Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105.
J Clin Invest. 1989 Jan;83(1):60-5. doi: 10.1172/JCI113885.
PTH was studied for its effects on bone formation in cultured rat calvariae. 0.01-10 nM PTH stimulated [3H]thymidine incorporation into DNA by up to 4.8-fold. Although continuous treatment with PTH for 24-72 h inhibited [3H]proline incorporation into collagen, transient (24 h) treatment enhanced [3H]proline incorporation into collagen 24-48 h after the hormone was removed. The collagen stimulated by PTH was type I and the effect was observed in the periosteum-free bone and was not blocked by hydroxyurea. Furthermore, treatment with 1-100 nM PTH for 24 h increased insulin-like growth factor (IGF) I concentrations by two to fourfold, and an IGF I antibody prevented the PTH stimulation of collagen synthesis, but not its mitogenic effect. In conclusion, continuous treatment with PTH inhibits calvarial collagen, whereas transient treatment stimulates collagen synthesis, and the stimulatory effect is mediated by local production of IGF I.
研究了甲状旁腺激素（PTH）对培养的大鼠颅骨骨形成的影响。0.01 - 10 nM的PTH可使[3H]胸腺嘧啶核苷掺入DNA的量增加达4.8倍。虽然用PTH连续处理24 - 72小时会抑制[3H]脯氨酸掺入胶原蛋白，但短暂（24小时）处理在去除激素后24 - 48小时可增强[3H]脯氨酸掺入胶原蛋白。PTH刺激产生的胶原蛋白为I型，在无骨膜的骨中观察到这种效应，且不受羟基脲的阻断。此外，用1 - 100 nM的PTH处理24小时可使胰岛素样生长因子（IGF）I的浓度增加两到四倍，并且IGF I抗体可阻止PTH对胶原蛋白合成的刺激作用，但不能阻止其促有丝分裂作用。总之，PTH连续处理会抑制颅骨胶原蛋白，而短暂处理则刺激胶原蛋白合成，且这种刺激作用是由局部产生的IGF I介导的。
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