Fernandes Gabriel R, Barbosa Aulus E A D, Almeida Renan N, Castro Fabíola F Dos S, da Ponte Marina de C P, Faria-Junior Celio, Müller Fernanda M P, Viana Antônio A B, Grattapaglia Dario, Franco Octavio L, Alencar Sérgio A, Dias Simoni C
Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil.
Hospital Santa Luzia, Brasília, Brazil.
Front Microbiol. 2017 Oct 23;8:1993. doi: 10.3389/fmicb.2017.01993. eCollection 2017.
, also known as group A (GAS), is a human pathogen that causes diverse human diseases including streptococcal toxic shock syndrome (STSS). A GAS outbreak occurred in Brasilia, Brazil, during the second half of the year 2011, causing 26 deaths. Whole genome sequencing was performed using Illumina platform. The sequences were assembled and genes were predicted for comparative analysis with type 1 strains: MGAS5005 and M1 GAS. Genomics comparison revealed one of the invasive strains that differ from others isolates and from 1 reference genomes. Also, the new invasive strain showed differences in the content of virulence factors compared to other isolated in the same outbreak. The evolution of contemporary GAS strains is strongly associated with horizontal gene transfer. This is the first genomic study of a outbreak in Brazil, and revealed the rapid bacterial evolution leading to new clones. The emergence of new invasive strains can be a consequence of the injudicious use of antibiotics in Brazil during the past decades.
A群链球菌，也称为A组链球菌（GAS），是一种人类病原体，可导致多种人类疾病，包括链球菌中毒性休克综合征（STSS）。2011年下半年，巴西首都巴西利亚发生了A群链球菌疫情，造成26人死亡。使用Illumina平台进行了全基因组测序。对序列进行了组装，并预测了基因，以便与1型菌株MGAS5005和M1 GAS进行比较分析。基因组学比较显示，其中一种侵袭性菌株与其他分离株以及1个参考基因组不同。此外，与同一疫情中的其他分离株相比，新的侵袭性菌株在毒力因子含量上也存在差异。当代A群链球菌菌株的进化与水平基因转移密切相关。这是巴西首次对A群链球菌疫情进行的基因组研究，揭示了导致新克隆的细菌快速进化。过去几十年巴西抗生素的不当使用可能导致了新侵袭性菌株的出现。
