O'Hara B P, Pyle J, McCarthy D, Archer J R
ARC Bone and Joint Research Unit, London Hospital Medical College, U.K.
J Immunol Methods. 1989 Jan 17;116(2):175-9. doi: 10.1016/0022-1759(89)90201-9.
Pepsinogen has previously been shown to bind non-specifically to immune complexes and aggregated immunoglobulins. We demonstrate here using a solid-phase immunoassay that immunoglobulins aggregated by heat or glutaraldehyde bind non-specifically to several different enzymes. Some of these, including pepsinogen (marketed as pepsin), hyaluronidase and trypsin, are used in the breakdown of tissues or biochemical preparations during the preparation of antigens. Contamination of impure antigens by enzyme is likely to lead to products which bind non-specifically to immune complexes. This can cause misidentification of complexes as antibodies. We recommend that all tests for specific antibody involving the use of antigens prepared by these or other enzymes should include a control with aggregated immunoglobulin substituted for the test serum.
胃蛋白酶原此前已被证明可非特异性地结合免疫复合物和聚集的免疫球蛋白。我们在此使用固相免疫测定法证明，经加热或戊二醛聚集的免疫球蛋白可非特异性地结合几种不同的酶。其中一些酶，包括胃蛋白酶原（作为胃蛋白酶销售）、透明质酸酶和胰蛋白酶，在抗原制备过程中用于组织分解或生化制剂处理。不纯抗原被酶污染很可能导致产物非特异性地结合免疫复合物。这可能会导致将复合物误鉴定为抗体。我们建议，所有涉及使用由这些酶或其他酶制备的抗原进行的特异性抗体检测，均应包括用聚集免疫球蛋白替代检测血清的对照。
