Bhattacharya A, Ranadive S N, Kale M, Bhattacharya S
Am Rev Respir Dis. 1986 Aug;134(2):205-9. doi: 10.1164/arrd.1986.134.2.205.
Immune complexes were isolated from sera of tuberculosis patients by precipitation with 2.5% polyethylene glycol. The precipitates were characterized by quantitative determination of different immunoglobulin classes by single radioimmunodiffusion, sodium dodecyl sulfate polyacrylamide gel electrophoresis for presence of serum components, Ouchterlony's double diffusion method for detection of complement components C3 and C4, and immuno-dot assay for detection of Mycobacterium tuberculosis antigens. The results showed that polyethylene glycol precipitates of patients' sera were indeed immune complexes, as they contained immunoglobulins, albumin, complement components, and mycobacterial antigens, whereas precipitates from control sera contained mainly albumin. The antibodies present in immune complexes were specific to M. tuberculosis antigen and showed no binding to Escherichia coli antigens. Immune complex levels, as determined by the ability to bind M. tuberculosis antigens in an enzyme-linked immunosorbent assay, were significantly higher in tuberculosis patients (n = 22) than in healthy control subjects (n = 18). Thus, immune complex level could be a useful parameter in the diagnosis of active tuberculosis.
通过用2.5%聚乙二醇沉淀法从肺结核患者血清中分离免疫复合物。通过单向放射免疫扩散法定量测定不同免疫球蛋白类别对沉淀物进行表征，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳检测血清成分的存在，用双向免疫扩散法检测补体成分C3和C4，并用免疫斑点法检测结核分枝杆菌抗原。结果表明，患者血清的聚乙二醇沉淀物确实是免疫复合物，因为它们含有免疫球蛋白、白蛋白、补体成分和分枝杆菌抗原，而对照血清的沉淀物主要含有白蛋白。免疫复合物中存在的抗体对结核分枝杆菌抗原具有特异性，与大肠杆菌抗原无结合。通过酶联免疫吸附测定法结合结核分枝杆菌抗原的能力确定，肺结核患者（n = 22）的免疫复合物水平显著高于健康对照受试者（n = 18）。因此，免疫复合物水平可能是活动性肺结核诊断中的一个有用参数。
