妊娠期糖尿病患者中长链非编码 RNA MALAT1 的表达。

Long non-coding RNA MALAT1 expression in patients with gestational diabetes mellitus.

机构信息

Department of Gynaecology and Obstetrics, Ningbo Medical Center Li Huili Eastern Hospital, Affiliated to Ningbo University, Ningbo, China.

Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, China.

出版信息

Int J Gynaecol Obstet. 2018 Feb;140(2):164-169. doi: 10.1002/ijgo.12384. Epub 2017 Nov 30.

DOI:10.1002/ijgo.12384

PMID:29110299

Abstract

OBJECTIVE

To monitor the expression of long non-coding RNAs (lncRNAs) and changes in metabolic profiles among patients with gestational diabetes mellitus (GDM).

METHODS

A retrospective case-control study was conducted using data for women treated at a hospital in China between October 8, 2014, and April 15, 2015. Patients diagnosed with GDM using an oral glucose tolerance test at 24-28 weeks of pregnancy (n=50) were included. The control group comprised pregnant women without GDM (n=47). Serum levels of lncRNA MALAT1, lncRNA p21, and lncRNA H19 were assessed using the reverse transcription polymerase chain reaction. Plasma glucose and lipid levels were assessed biochemically.

RESULTS

The expression level of lncRNA MALAT1 was higher among the cases than the controls (P=0.007). Expression of IncRNA MALAT1 among the patients with GDM correlated with both lncRNA p21 (r=0.333, P=0.018) and lncRNA H19 (r=0.314, P=0.030). Levels of triglycerides and total cholesterol differed between the control group and patients with GDM (3.6 ± 2.6 mmol/L vs 3.8 ± 1.5 mmol/L and 6.54 ± 1.30 mmol/L vs 7.40 ± 0.90 mmol/L, respectively; P<0.05). The area under the receiver operating characteristic curve was 0.654 (95% confidence interval 0.543-0.768, P<0.001).

CONCLUSION

Expression of lncRNA MALAT1 could offer a novel biomarker to predict GDM.

摘要

目的

监测妊娠糖尿病（GDM）患者长链非编码 RNA（lncRNA）的表达和代谢谱变化。

方法

采用回顾性病例对照研究，纳入 2014 年 10 月 8 日至 2015 年 4 月 15 日在中国一家医院接受治疗的女性患者的数据。通过 24-28 周妊娠口服葡萄糖耐量试验诊断为 GDM 的患者（n=50）纳入病例组。对照组为无 GDM 的妊娠妇女（n=47）。采用逆转录聚合酶链反应检测血清 lncRNA MALAT1、lncRNA p21 和 lncRNA H19 水平。采用生化方法检测血浆葡萄糖和血脂水平。

结果

病例组中 lncRNA MALAT1 的表达水平高于对照组（P=0.007）。GDM 患者 lncRNA MALAT1 的表达与 lncRNA p21（r=0.333，P=0.018）和 lncRNA H19（r=0.314，P=0.030）均相关。对照组和 GDM 患者的甘油三酯和总胆固醇水平存在差异（3.6±2.6mmol/L 比 3.8±1.5mmol/L 和 6.54±1.30mmol/L 比 7.40±0.90mmol/L，P<0.05）。受试者工作特征曲线下面积为 0.654（95%置信区间 0.543-0.768，P<0.001）。