Shinohara T, Ohta Y, Kawate N, Takahashi M, Sakagami N, Inaba T, Tamada H
Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
Reprod Domest Anim. 2018 Feb;53(1):270-273. doi: 10.1111/rda.13096. Epub 2017 Nov 6.
This study examined the effects of treatment with U0126, which inhibits MAPK by inhibiting MAPK kinase, during the first 2 hr of in vitro maturation on bovine developmental competence and on gap junction (GAPJ) communication between the oocyte and cumulus cells. The percentage of oocytes developing to the blastocyst stage in the group treated with 5 μM U0126 (28%) was significantly higher than that in controls (15%, p < .05), while that in the group treated with 10 μM U0126 (18%) was not. Breakdown of the GAPJs was delayed in the group treated with 5 μM U0126 when compared to controls, as estimated by immunohistochemical examination of connexin 43, which is a primary constituent of the GAPJs. These results indicate that treatment with 5 μM U0126 during in vitro maturation delays GAPJ breakdown and improves bovine oocyte developmental competence.
本研究检测了在体外成熟的最初2小时内，用U0126（通过抑制丝裂原活化蛋白激酶激酶来抑制丝裂原活化蛋白激酶）处理对牛的发育能力以及卵母细胞与卵丘细胞间缝隙连接（GAPJ）通讯的影响。用5μM U0126处理的组中发育至囊胚阶段的卵母细胞百分比（28%）显著高于对照组（15%，p < 0.05），而用10μM U0126处理的组中（18%）则不然。通过对缝隙连接主要成分连接蛋白43进行免疫组织化学检测估计，与对照组相比，用5μM U0126处理的组中缝隙连接的解体延迟。这些结果表明，体外成熟期间用5μM U0126处理可延迟缝隙连接解体并提高牛卵母细胞的发育能力。
