a Department of Laboratory Diagnosis , Jinzhou Medical University , Jinzhou , PR China.
b Department of Basic Nursing , Jinzhou Medical University , Jinzhou , PR China.
Pharm Biol. 2017 Dec;55(1):2170-2177. doi: 10.1080/13880209.2017.1396350.
Momordica charantia L. (Cucurbitaceae) has long been widely used as a traditional remedy for diabetes mellitus in some countries. However, detailed antidiabetic mechanisms are largely unknown.
This study clarified the ameliorating effects of M. charantia ethanol extracts (MCE) on the insulin resistance in type 2 diabetes mellitus (T2DM) rats.
T2DM rat model was established by high-fat diet and streptozotocin (STZ) injection. Diabetic rats were randomized into five groups: the model control group (n = 8) (common diet), the high-fat diet metformin (50 mg/kg/d), and the three-dose MCE (100, 200, and 400 mg/kg/d) groups (n = 8 each). After 8 weeks, the fasting serum glucose, insulin, TNF-α, and IL-6 were measured, and the relevant factors of glucose and insulin were monitored by glycogen dyeing, RT-PCR, and western blot, respectively.
The 8-week treatment of 400 mg/kg MCE significantly lowered body weight (330.1 versus 365.9 g), serum glucose (7.41 versus 16.63 mmol/L), insulin (12.06 versus 15.89 mIU/L), TNF-α (52.72 versus 81.83 ng/L), and IL-6 (104.81 versus 135.74 ng/L) in comparison with those of the diabetic control group (p < 0.05). It was the same for skeletal muscle glucose transporter 4 (GLUT-4) protein, and glycogen level, suppressor of cytokine signaling-3 (SOCS-3), c-Jun N-terminal kinase (JNK), and Akt expression at both protein and mRNA levels in liver (p < 0.05).
MCE can ameliorate insulin resistance in T2DM rats. This effect may be related to the regulation of mRNA and protein levels of SOCS-3 and JNK.
苦瓜 L.（葫芦科）长期以来一直被广泛用作某些国家治疗糖尿病的传统药物。然而，详细的抗糖尿病机制在很大程度上尚不清楚。
本研究阐明苦瓜乙醇提取物（MCE）对 2 型糖尿病（T2DM）大鼠胰岛素抵抗的改善作用。
采用高脂饮食和链脲佐菌素（STZ）注射建立 T2DM 大鼠模型。糖尿病大鼠随机分为五组：模型对照组（n=8）（普通饮食）、高脂饮食二甲双胍（50mg/kg/d）和三个剂量 MCE（100、200 和 400mg/kg/d）组（n=8 组）。8 周后，测定空腹血清葡萄糖、胰岛素、TNF-α和 IL-6，并分别通过糖原染色、RT-PCR 和 Western blot 监测葡萄糖和胰岛素的相关因素。
400mg/kg MCE 治疗 8 周可显著降低体重（330.1 与 365.9g）、血清葡萄糖（7.41 与 16.63mmol/L）、胰岛素（12.06 与 15.89mIU/L）、TNF-α（52.72 与 81.83ng/L）和 IL-6（104.81 与 135.74ng/L），与糖尿病对照组相比差异有统计学意义（p<0.05）。骨骼肌葡萄糖转运蛋白 4（GLUT-4）蛋白和糖原水平、细胞因子信号转导抑制因子-3（SOCS-3）、c-Jun N-末端激酶（JNK）和 Akt 的表达在肝脏中也有相同的作用，无论是在蛋白水平还是在 mRNA 水平上（p<0.05）。
MCE 可改善 T2DM 大鼠的胰岛素抵抗。这种作用可能与 SOCS-3 和 JNK 的 mRNA 和蛋白水平的调节有关。
