Seifi Sima, Nazari Seyedeh Elnaz, Avan Amir, Khalili-Tanha Nima, Babaei Fatemeh, Soleimanpour Saman, Asgharzadeh Fereshteh, Hajzadeh Mousa-Al-Reza, Khazaei Majid, Marjani Abdoljalal
Metabolic Disorders Research Center, Department of Biochemistry and Biophysics, Golestan University of Medical Sciences Gorgan, Golestan, Iran.
Metabolic syndrome Research center, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2024 May-Jun;14(3):388-401. doi: 10.22038/AJP.2024.24011.
Muscle atrophy due to immobility is a common complication of many diseases and a consequence of therapeutic processes. Immobility and inactivity have been shown to be associated with increased inflammation. The aim of this study was to investigate the therapeutic potential of Wild Bitter Melon (WBM) (Momordica charantia Linn) on muscle atrophy due to immobility in a mouse model.
This study was performed in two phases of atrophy and recovery on male BALB/c mice which were divided into 3 groups: control, immobilized, and experimental. The treatment period with WBM at a dose of 400 mg/kg daily by gavage was 17 days, including 7 days of being immobilized and 10 days of recovery. At the end of each phase, half of the mice from each group were examined regarding the four limb grip strength, and then histological and biochemical analyses were done.
The tissue level of malondialdehyde (MDA) oxidative stress index in the atrophy phase in the atrophy group (5.4567±0.522) nmol/g compared to the control group (3.455±0.065) nmol significantly (p 0.001) <) increased. Also, the tissue level of MDA in the WBM group (3.87±0.035) showed a significant decrease compared to the atrophy group (p<0.01). The strength percentage of four limbs in the mice of the treatment group (-23.46±2.45) was significantly higher than that of the atrophy group (-30.60±3.15) at the end of the atrophy phase.
The results suggest that the use of WBM reduces the degree of inflammation, oxidative stress and muscle damage, as well as muscle atrophy, which may improve the muscle atrophy in mice.
因活动受限导致的肌肉萎缩是许多疾病常见的并发症以及治疗过程的后果。已表明活动受限和不活动与炎症增加有关。本研究的目的是在小鼠模型中研究野生苦瓜（WBM）（苦瓜）对因活动受限导致的肌肉萎缩的治疗潜力。
本研究在雄性BALB/c小鼠上进行了萎缩和恢复两个阶段，将其分为3组：对照组、固定组和实验组。通过灌胃以每日400mg/kg的剂量给予WBM的治疗期为17天，包括7天的固定期和10天的恢复期。在每个阶段结束时，对每组一半的小鼠进行四肢握力检查，然后进行组织学和生化分析。
萎缩组在萎缩阶段丙二醛（MDA）氧化应激指数的组织水平（5.4567±0.522）nmol/g与对照组（3.455±0.065）nmol相比显著（p<0.001）升高。此外，WBM组的MDA组织水平（3.87±0.035）与萎缩组相比显著降低（p<0.01）。在萎缩阶段结束时，治疗组小鼠四肢的力量百分比（-23.46±2.45）显著高于萎缩组（-30.60±3.15）。
结果表明，使用WBM可降低炎症程度、氧化应激和肌肉损伤以及肌肉萎缩，这可能改善小鼠的肌肉萎缩。
