巴西伯克霍尔德菌假鼻疽亚种的临床和环境分离株：基因分型及毒力基因检测

Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene.

作者信息

Bandeira Tereza de Jesus Pinheiro Gomes, Castelo-Branco Débora de Souza Collares Maia, Rocha Marcos Fábio Gadelha, Cordeiro Rossana de Aguiar, Ocadaque Crister José, Paiva Manoel de Araújo Neto, Brilhante Raimunda Sâmia Nogueira, Sidrim José Júlio Costa

机构信息

Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil; Christus School of Medicine, Fortaleza, Ceará, Brazil.

Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, Brazil.

出版信息

Asian Pac J Trop Med. 2017 Oct;10(10):945-951. doi: 10.1016/j.apjtm.2017.09.004. Epub 2017 Sep 18.

DOI:10.1016/j.apjtm.2017.09.004

PMID:29111189

Abstract

OBJECTIVE

To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará, Brazil, and screen these isolates for the presence of type three secretion system virulence gene.

METHODS

Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction.

RESULTS

Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates.

CONCLUSIONS

This is an effort to type B. pseudomallei strains from Ceará, which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.

摘要

目的

评估在巴西塞阿拉州分离出的临床和环境来源的类鼻疽伯克霍尔德菌（B. pseudomallei）的遗传多样性，并筛查这些分离株中是否存在三型分泌系统毒力基因。

方法

分析了19株类鼻疽伯克霍尔德菌分离株（9株来自临床病例，10株来自土壤）。使用引物OPQ - 2、OPQ - 4和OPQ - 16进行随机扩增多态性DNA分析以评估遗传多样性，并通过聚合酶链反应检测三型分泌系统基因。

结果

随机扩增多态性DNA显示，在测试的类鼻疽伯克霍尔德菌分离株中，遗传相关性约为50%，这些分离株被分为两个进化枝，其中最大的进化枝对于引物OPQ - 2包含18/19个分离株，对于引物OPQ - 16包含17/19个分离株。引物OPQ - 4将分离株分为三个进化枝，分别包含1/19、3/19和15/19个分离株。此外，在所有测试分离株中均检测到三型分泌系统基因。