Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, Brazil.
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, Brazil.
J Glob Antimicrob Resist. 2017 Dec;11:133-137. doi: 10.1016/j.jgar.2017.10.014. Epub 2017 Oct 31.
Bacteria belonging to the genera Ochrobactrum and Achromobacter are bacteria considered opportunistic, causing infections mainly in immunocompromised patients. β-lactamases are the main cause of resistance to β-lactam antibiotics. This study aimed to investigate the antimicrobial resistance profile and the presence of β-lactamases encoding genes in Ochrobactrum sp. and Achromobacter sp. isolated from Brazilian soils.
Soil samples from the five regions of Brazil were collected for the isolation of bacteria, which were identified molecularly and then, the minimum inhibitory concentration and detection of β-lactamases encoding genes were performed.
High-level of resistance to β-lactam antibiotics and different β-lactamases encoding genes were found (bla, bla, bla and bla), including the first report of the presence of bla in bacteria belonging to the genera Ochrobactrum and Achromobacter.
The results showed that the bacteria from this study, belonging to genera Ochrobactrum and Achromobacter isolated from soil, harbor different β-lactamases encoding genes and can act as a reservoir of these genes.
属于奥克霍布鲁克氏菌属和无色杆菌属的细菌被认为是机会致病菌，主要在免疫功能低下的患者中引起感染。β-内酰胺酶是导致β-内酰胺类抗生素耐药的主要原因。本研究旨在调查从巴西土壤中分离出的奥克霍布鲁克氏菌属和无色杆菌属的抗菌药物耐药谱和β-内酰胺酶编码基因的存在情况。
从巴西的五个地区采集土壤样本进行细菌分离，通过分子鉴定，然后进行最低抑菌浓度测定和β-内酰胺酶编码基因检测。
发现这些细菌对β-内酰胺类抗生素高度耐药，并存在不同的β-内酰胺酶编码基因（blaCTX-M-15、blaOXA-1、blaOXA-23 和 blaOXA-48），包括首次报道奥克霍布鲁克氏菌属和无色杆菌属细菌中存在 blaCTX-M-15 基因。
研究结果表明，从土壤中分离出的属于奥克霍布鲁克氏菌属和无色杆菌属的这些细菌携带不同的β-内酰胺酶编码基因，可能成为这些基因的储存库。
