Furlan João Pedro Rueda, Stehling Eliana Guedes
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N. Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil.
Environ Monit Assess. 2018 Jan 10;190(2):76. doi: 10.1007/s10661-017-6453-x.
β-lactam antibiotics are widely used for the treatment of different types of infections worldwide and the resistance to these antibiotics has grown sharply, which is of great concern. Resistance to β-lactams in gram-negative bacteria is mainly due to the production of β-lactamases, which are classified according to their functional activities. The aim of this study was to verify the presence of β-lactamases encoding genes in feces, soil, and water from a Brazilian pig farm. Different β-lactamases encoding genes were found, including bla, bla, bla, bla, bla, and bla. The bla and bla genes have been detected in all types of samples, indicating the spread of β-lactam resistant bacteria among farm pigs and the environment around them. These results indicate that β-lactamase encoding genes belonging to the cloxacillinase, ESBL, and carbapenemase and they have high potential to spread in different sources, due to the fact that genes are closely related to mobile genetic elements, especially plasmids.
β-内酰胺类抗生素在全球范围内被广泛用于治疗不同类型的感染，而对这些抗生素的耐药性急剧上升，这引起了极大关注。革兰氏阴性菌对β-内酰胺类抗生素的耐药性主要归因于β-内酰胺酶的产生，β-内酰胺酶根据其功能活性进行分类。本研究的目的是验证巴西一个养猪场的粪便、土壤和水中β-内酰胺酶编码基因的存在情况。发现了不同的β-内酰胺酶编码基因，包括bla、bla、bla、bla、bla和bla。bla和bla基因在所有类型的样本中均被检测到，这表明β-内酰胺耐药菌在猪场猪及其周围环境中传播。这些结果表明，属于氯唑西林酶、超广谱β-内酰胺酶和碳青霉烯酶的β-内酰胺酶编码基因，由于这些基因与移动遗传元件特别是质粒密切相关，它们在不同来源中有很高的传播潜力。
