Pavlova Anna S, Dyudeeva Evgeniya S, Kupryushkin Maxim S, Amirkhanov Nariman V, Pyshnyi Dmitrii V, Pyshnaya Inna A
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russian Federation.
Department of Natural sciences, Novosibirsk State University, Novosibirsk, Russian Federation.
Electrophoresis. 2018 Feb;39(4):670-674. doi: 10.1002/elps.201700415. Epub 2017 Nov 29.
SDS-PAGE is considered to be a universal method for size-based separation and analysis of proteins. In this study, we applied the principle of SDS-PAGE to the analysis of new entirely uncharged nucleic acid (NA) analogues, - phosphoryl guanidine oligonucleotides (PGOs). The procedure was also shown to be suitable for morpholino oligonucleotides (PMOs) and peptide nucleic acids (PNAs). It was demonstrated that SDS can establish hydrophobic interactions with these types of synthetic NAs, giving them a net negative charge and thus making these molecules mobile in polyacrylamide slab gels under the influence of an electric field.
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）被认为是一种基于大小对蛋白质进行分离和分析的通用方法。在本研究中，我们将SDS-PAGE原理应用于全新的完全不带电荷的核酸（NA）类似物——磷酸胍寡核苷酸（PGO）的分析。该方法也适用于吗啉代寡核苷酸（PMO）和肽核酸（PNA）。结果表明，SDS可与这些类型的合成核酸建立疏水相互作用，赋予它们净负电荷，从而使这些分子在电场作用下在聚丙烯酰胺平板凝胶中移动。
