利用2,5-二氧戊基（DOP）功能实现无触发生物正交邻近诱导的肽核酸连接

Triggerless Bio-Orthogonal Proximity-Induced PNA Ligation Using 2,5-Dioxopentanyl (DOP) Functionality.

作者信息

Manicardi Alex, Madder Annemieke

机构信息

Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.

Department of Organic and Macromolecular Chemistry, Organic and Biomimetic Chemistry Research Group, Ghent University, Ghent, Belgium.

出版信息

Methods Mol Biol. 2025;2901:227-239. doi: 10.1007/978-1-0716-4394-5_17.

DOI:10.1007/978-1-0716-4394-5_17

PMID:40175879

Abstract

Peptide nucleic acids (PNAs) are emerging tools in the construction of nanostructures possessing self-assembly properties similar to those of DNA but featuring enhanced stability and chemical flexibility. Templated-ligation approaches can be exploited to stabilize the formed nanoproducts, with bio-orthogonal methods preferred for compatibility with natural components. This chapter outlines design requirements and protocols for conducting triggerless bio-orthogonal PNA ligation in solution and on glass surfaces, along with an electrophoretic monitoring protocol for assessing the outcome of solution ligation.

摘要

肽核酸（PNA）是构建纳米结构的新兴工具，其具有类似于DNA的自组装特性，但稳定性更高且化学灵活性更强。可以利用模板连接方法来稳定形成的纳米产物，其中生物正交方法因与天然成分的兼容性而更受青睐。本章概述了在溶液和玻璃表面进行无触发生物正交PNA连接的设计要求和方案，以及用于评估溶液连接结果的电泳监测方案。