Guli C L, Smyth D R
Department of Genetics, Monash University, Clayton, Vic., Australia.
Mutat Res. 1989 Feb;210(2):323-8. doi: 10.1016/0027-5107(89)90093-6.
Oocytes at the dictyate stage from young (8-14 weeks) and old (12-15 months) BALB/c mice were manually isolated and UV-irradiated. They were cultured for 1 h in medium containing tritiated thymidine and chased for a further hour in cold thymidine medium before being incubated for 18-20 h in medium with no added thymidine. Oocytes which had developed to metaphase II were analysed following autoradiography. Pooled results from 14 replicate experiments revealed no significant age-related difference between the mean corrected grain count per cell [159.2 +/- 8.5 (86 cells) for young mice and 164.6 +/- 9.8 (70 cells) for the old animals]. Thus in the female mouse the oocyte's capacity to repair UV-induced damage is apparently maintained at a high level throughout reproductive life.
从年轻（8 - 14周）和年老（12 - 15个月）的BALB/c小鼠中手动分离处于双线期的卵母细胞，并进行紫外线照射。将它们在含有氚标记胸腺嘧啶核苷的培养基中培养1小时，然后在冷胸腺嘧啶核苷培养基中再追踪1小时，之后在不添加胸腺嘧啶核苷的培养基中孵育18 - 20小时。对发育至减数分裂中期II的卵母细胞进行放射自显影分析。14次重复实验的汇总结果显示，每细胞平均校正颗粒计数在年轻小鼠[159.2±8.5（86个细胞）]和年老动物[164.6±9.8（70个细胞）]之间没有显著的年龄相关差异。因此，在雌性小鼠中，卵母细胞修复紫外线诱导损伤的能力在整个生殖生命过程中显然维持在较高水平。
