紫外线诱导的DNA修复在小鼠的前盘状卵母细胞中无法检测到。

UV-induced DNA repair is not detectable in pre-dictyate oocytes of the mouse.

作者信息

Guli C L, Smyth D R

机构信息

Department of Genetics, Monash University, Clayton, Vic., Australia.

出版信息

Mutat Res. 1988 Jun;208(2):115-9. doi: 10.1016/s0165-7992(98)90010-0.

DOI:10.1016/s0165-7992(98)90010-0

PMID:3380109

Abstract

Oocytes from fetal and neonatal mice were UV-irradiated, cultured in medium containing tritiated thymidine and analysed following autoradiography. As previously reported irradiated dictyate cells clearly displayed an increased grain count. However we detected no UV-induced response in oocytes at the leptotene, zygotene or pachytene stages of meiosis. This contrasts with the situation in spermatogenesis where high levels of repair can be induced in equivalent early prophase stages.

摘要

对来自胎儿和新生小鼠的卵母细胞进行紫外线照射，在含有氚化胸腺嘧啶核苷的培养基中培养，并在放射自显影后进行分析。如先前报道，受照射的双线期细胞的银粒计数明显增加。然而，我们在减数分裂细线期、偶线期或粗线期的卵母细胞中未检测到紫外线诱导的反应。这与精子发生的情况形成对比，在精子发生过程中，在相同的早期前期阶段可诱导高水平的修复。