Dong Fengyun, Wang Jin, Xu Yinghua, Cheng Zuowang, Chen Xiaocui, Wang Yifan, Liu Ju
Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China.
School of Basic Medical Sciences, Shandong University, Jinan, Shandong 250012, P.R. China.
Oncol Lett. 2017 Nov;14(5):6012-6016. doi: 10.3892/ol.2017.6975. Epub 2017 Sep 15.
Cluster of differentiation (CD) 109 is a glycosylphosphatidylinositol-anchored cell surface glycoprotein and a co-receptor for transforming growth factor β. The expression of CD109 has been detected in squamous cell carcinoma of the lung, esophagus, skin and gallbladder. The aim of the present study was to evaluate CD109 expression in penile squamous cell carcinoma (PSCC). CD109 expression in PSCC tumor and adjacent tissues from 45 specimens in tissue microarrays was examined by immunohistochemical analyses. In addition, 3 fresh surgical samples of PSCC were collected and examined for their CD109 mRNA and protein levels by reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence staining. CD109 transcription and expression were significantly higher in the PSCC tissues compared with adjacent normal penile tissues, and its expression was restricted to squamous cells. However, CD109 expression level was not associated with the PSCC differentiation grade. These results suggest that CD109 may be associated with the pathogenesis of PSCC, and may therefore be a potential therapeutic target.
分化簇（CD）109是一种糖基磷脂酰肌醇锚定的细胞表面糖蛋白，也是转化生长因子β的共受体。已在肺癌、食管癌、皮肤癌和胆囊癌的鳞状细胞癌中检测到CD109的表达。本研究的目的是评估CD109在阴茎鳞状细胞癌（PSCC）中的表达。通过免疫组织化学分析检测组织微阵列中45个样本的PSCC肿瘤组织和相邻组织中的CD109表达。此外，收集了3份PSCC新鲜手术样本，通过逆转录定量聚合酶链反应、蛋白质免疫印迹和免疫荧光染色检测其CD109 mRNA和蛋白水平。与相邻的正常阴茎组织相比，PSCC组织中的CD109转录和表达显著更高，且其表达仅限于鳞状细胞。然而，CD109表达水平与PSCC分化程度无关。这些结果表明，CD109可能与PSCC的发病机制有关，因此可能是一个潜在的治疗靶点。
